和营安心方抗炎及促进血管再生作用的研究

Protective effect of Heying Anxin formula on anti-inflammatory and promoting angiogenesis

关键词:  和营安心方  血管平滑肌细胞  老化大鼠  心血管系统

Key words:  Heying Anxin formula  vascular smooth muscle cells  aging rats  cardiovascular system

摘要:

[目的] 研究和营安心方对老化大鼠心血管系统的保护作用。[方法] 采用过氧化氢（H₂O₂）建立大鼠血管平滑肌细胞炎症损伤模型，实验分为5组，正常对照组、H₂O₂（200 μmol/L）模型组、和营安心方高、中、低剂量组，分别加入“和营安心方”提取物100、50、25 mg/L，24 h之后加入与模型组同浓度的H₂O₂（200 μmol/L），共同作用2 h，观察血管平滑肌细胞培养液中一氧化氮（NO）含量。以大鼠颈背部皮下注射125 mg/kg D-半乳糖建立大鼠老化模型，用实时荧光定量聚合酶链式反应（PCR）法检测大鼠脑组织及肝脏组织中一氧化氮合酶（NOS）及碱性成纤维因子（b-FGF）的表达，实验分为6组，分别为正常组、模型组（D-半乳糖125 mg/kg）、和营安心方高剂量组（500 mg/kg+D-半乳糖125 mg/kg）、和营安心方中剂量组（250 mg/kg+D-半乳糖125 mg/kg）、和营安心方低剂量组（125 mg/kg+D-半乳糖125 mg/kg）及青年大鼠组。[结果] 和营安心方能显著性降低大鼠血管平滑肌细胞上清液中NO的含量，与模型组比较差异有统计学意义（P<0.05），实时荧光定量PCR法测定结果显示，相对于模型组，和营安心方能显著性降低大鼠脑组织和肝脏组织中NOS的表达，提高b-FGF在大鼠脑及肝脏组织中的表达（P<0.01）。[结论] 和营安心方对老化大鼠的心血管再生及抗炎等方面有一定程度的保护作用，提示和营安心方可能通过直接作用于脑及肝脏组织来发挥促进血管再生及抗炎的功效。

Abstract:

[Objective] To investigate protective effects and mechanisms of Heying Anxin formula on the cardiovascular system of aging rats. [Methods] Rat vascular smooth muscle cells were incubated by H₂O₂(200 μmol/L) for 2 h as inflammation model group, same dose of H₂O₂ was administered 2 h after treatment with Heying An xin formula(100 μg/mL, 50 μg/mL, 25 μg/mL)as therapeutic group and incubated for another 24 h, content of NO were measured. Subcutaneous injection in neck and back of D-galactose(125 mg/kg) to establish aging rats model. Model group: D-galactose(125 mg/kg), Heying Anxin formula (500 mg/kg+D-galactose 125 mg/kg, 250 mg/kg+D-galactose 125 mg/kg、125mg/kg+D-galactose 125 mg/kg) as treating groups, as well as normal group and young group. The RT-PCR technique was used to measure the expression of relevant genes, such as nitrous oxide system(NOS) and basic fibroblast growth factor (b-FGF) in the tissues of brain and liver. [Results] Heying Anxin formula can significantly decrease the content of NO(P<0.05 vs control group), also compared with that of control group, He ying Anxin formula can significantly decrease NOS expression and enhance b-FGF expression in rats brain and liver tissues (P<0.01). [Conclusion] Heying Anxin formula has greatly protective effects on anti-inflammatory and cardiovascular regeneration of aging rats, and it may be take effect by the brain and liver.