芪参益气滴丸对培养大鼠主动脉环血管新生及稳定的影响

Effects of Qishen Yiqi pill on angiogenesis of cultured rat aortic ring

关键词:  芪参益气滴丸  主动脉环  血管新生及稳定

Key words:  Astragalus and ginseng  the dropping pill  aortic ring  angiogenesis and stability

摘要:

[目的] 探讨芪参益气滴丸对培养大鼠主动脉环血管新生及稳定的影响。[方法] 三维培养大鼠主动脉环，分为正常组、血管内皮生长因子（VEGF）组、芪参益气滴丸组、VEGF+芪参益气滴丸组，分别加入正常培养基及分别含10 μg/L VEGF、25 mg/L芪参益气滴丸、10 μg/L VEGF+25 mg/L芪参益气滴丸的正常培养基，每组3复孔。从第3天开始，隔天计数新生血管的总分支数、总长度、平均长度、最长长度，直至新生血管退化，比较各组对血管新生及稳定的影响。[结果] 正常组主动脉环前3 d血管逐渐长出，第3～7天新生血管长度增加，数量变化不明显，7 d后，新生血管数量和长度均降低，此后逐渐退化；与正常组比较，各用药组均能促进新生血管发芽（1～3 d）及3~7 d持续生长。7 d后，虽各组新生血管总分支数（除芪参益气滴丸组第13～15天外）、总长度、平均长度和最长长度较正常组均有增加趋势，但单用芪参益气滴丸则血管迅速退化，单用VEGF血管数量及长度不再明显增加，且新生血管结构在第11天明显破坏，并逐步退化，而芪参益气与VEGF滴丸合用能使新生血管在第7～13天持续稳定生长，并延缓其退化。[结论] 芪参益气滴丸能促进三维培养主动脉环血管新生，但维持时间较短，与VEGF合用有协同作用，可促进血管新生，维持持续生长较长时间，并延缓新生血管退化。

Abstract:

[Objective] To investigate the effect of Qishenyiqi pill on the angiogenesis of cultured rat aortic ring. [Methods] Three dimensional culture of rat aortic rings in 48 wells plate, divided them into four groups: normal group(culture with normal medium), VEGF group(culture with normal medium with 10 ng/ml VEGF), Qishenyiqi pill group(culture with normal medium with 25 μg/mL QiShenYiQi pills), and Qishenyiqi pill with VEGF group(culture with normal medium with 25 mg/L Qishenyiqi pills and 10 μg/L VEGF). Every group had three wells. Change the medium every three days. Record the number of the total number, total length, average length, the longest length of the microvessels which grow out of the aortic ring every two days from the 3rd day after culture until the neovascularization degradation. [Results] In the normal group, microvessels gradually grow out from the aortic ring in 1~3 days, and continue grow longer and longer in the 3~7 days, but the number of neovasculare was the same with day 3. Then number and length of the neovasculare decreased after 7 days, in the other word, the neovasculare gradually degraded. Compared with the normal group, the treatment group can promote angiogenesis germination (1~3 days) and growth in 3~7 days. But after 7 days, although the total number (except Qishen Yiqi pill group 13~15 days), total length and average length and the longest length of the new microvascular was increased than that of the normal group, but the new vascular rapidly degraded in the Qishen Yiqi pill group, in the VEGF group the number and length of the neovasculare was not obviously increased and the structure of new vessels in the day 11 was destroyed and degenerated and gradually, in the Qishen Yiqi combined the VEGF group the length of neovasculare was increased continully in 7~13 days, indicated that the angiogenesis in this group was increased in 7~13 days sustained. [Conclusion] Qishen Yiqi pills can promote angiogenesis of the three-dimensional culture of aortic ring angiogenesis, but this affection maintained for a short time, if combined with VEGF it can promote angiogenesis and maintain sustained growth for a long time, and delay the degradation of angiogenesis.