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| HS-SPEM-GC-MS分析丹蒌片中的挥发性化学成分 |
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张娜1,2, 李琳1, 高杉1, 朱彦1, 王家明3, 张鹏1, 于春泉1
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1.天津中医药大学, 天津市现代中药重点实验室, 天津 300193;2.天津国际生物医药联合研究院, 中药新药研发中心, 天津 300457;3.天津科技大学, 生物工程学院, 天津 300457
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| 摘要: |
| [目的] 用气相色谱-质谱联用（GC-MS）技术鉴定丹蒌片挥发性化学成分。[方法] 采用顶空固相微萃取法（HS-SPEM）提取丹蒌片中的挥发性成分，利用GC-MS 结合保留指数法（KI）对其主要挥发性成分进行分析鉴定。[结果] 从丹蒌片挥发性成分中鉴定出44个组分，占总挥发性组分的72.46%.其中相对含量超过 5% 的成分有蒿本内酯（17.49%）,β-榄香烯（11.07%）,（-）斯巴醇（8.95%）,3-丁烯基-1（3H）-异苯并呋喃酮（5.50%）等6个化合物。[结论] 丹蒌片中的挥发性成分得到全面分析，根据其相对含量，丹蒌片中的挥发性成分主要来源于川芎、郁金、泽泻，为丹蒌片的质量控制和进一步药效研究提供一定的参考。 |
| 关键词: 丹蒌片 挥发油 GC-MS分析 |
| DOI：10.11656/j.issn.1673-9043.2016.04.10 |
| 分类号: |
| 基金项目:国家自然科学基金青年项目（81303183，81403198）;国家重点基础研究发展计划（“973”计划）项目（2014CB542902）。 |
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| HS-SPEM-GC-MS analysis of the volatile components of Dan-lou tablets |
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ZHANG Na1,2, LI Lin1, GAO Shan1, ZHU Yan1, WANG Jia-ming3, ZHANG Peng1, YU Chun-quan1
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1.Tianjin University of Traditional Chinese Medicine, Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China;2.Tianjin International Joint Academy of Biomedicine, Research and Development Center of Traditional Chinese Drug, Tianjin 300457, China;3.Tianjin University of Science and Technology, Engineering College of Biology, Tianjin 300457, China
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| Abstract: |
| [Objective] Using gas chromatography-mass spectrometry technology(GC-MS) to identify the chemical composition of volatile oil of Dan-lou tablets. [Method] In this paper, volatile components of Dan-lou tablets were extracted by headspace solid phase microextraction (HS-SPEM). Their structures were identified based GC-MS database combined with Retention Index (KI) calculated. [Results] Fourty-four kinds of volatile oil compounds were identified from Dan-lou tablet, which accounted 72.46% of the total volatile oil components. The ingredients which have content more than 5% were Ligustilide (17.49%), β-Elemenone (11.07%), (-)-Spathulenol(8.95%), 3-butenyl-1(3H)-isobenzofuranone (5.502%) et al. [Conclusion] Volatile components of Dan-lou tablet are fully analyzed. The identifiedcompounds have multiple activities according the prevent reports. This article provides a scientific basis for quality control and further pharmacological research of Dan-lou tablets. |
| Key words: Dan-lou tablet volatile oil GC-MS analysis |
