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| 基于尤斯灌流室技术的泽泻汤大鼠肠吸收研究 |
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朱蕴1,2, 顾星1,2, 张兵1,2, 祁东利1,2, 刘志东1,2, 魏子斌1,2
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1.天津中医药大学, 天津市现代中药重点实验室-省部共建国家重点实验室培育基地, 天津 300193;2.天津中医药大学, 现代中药发现与制剂技术教育部工程研究中心, 天津 300193
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| 摘要: |
| [目的] 研究泽泻汤中成分白术内酯Ⅱ和Ⅲ在大鼠不同肠段的跨膜吸收特征。[方法] 采用Agilent ZorbaxPlus C18色谱柱，流动相为乙腈-0.1%甲酸水（42：58），流速：0.3 mL/min。质谱电喷雾离子源，阳离子MRM模式，白术内酯Ⅱ和Ⅲ的定量离子分别为m/z233.2→m/z187，m/z249.15→m/z231。尤斯灌流室法考察泽泻汤在大鼠离体十二指肠、空肠、回肠、结肠的吸收，接收侧样品10000 r/min离心15 min后用液相色谱-质谱色谱法（LC-MS/MS）分析。[结果] 白术内酯Ⅱ在大鼠不同肠黏膜的表观渗透系数范围在6.10~25.22×10-6 cm/s之间，白术内酯Ⅲ在1.88~5.41×10-6cm/s之间，两者最佳吸收部位是结肠。[结论] 该方法专属性好，准确度高，可用于泽泻汤中白术内酯Ⅱ和Ⅲ的大鼠肠吸收研究。 |
| 关键词: 泽泻汤 尤斯灌流室 LC-MS/MS 白术内酯Ⅱ 白术内酯 |
| DOI：10.11656/j.issn.1673-9043.2017.04.16 |
| 分类号:R285.5 |
| 基金项目:新世纪优秀人才支持计划（NCET-12-1068）。 |
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| Rat intestinal absorption study of Zexie decoction with Ussing chamber by UPLC-MS/MS |
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ZHU Yun1,2, GU Xing1,2, ZHANG Bing1,2, QI Dong-li1,2, LIU Zhi-dong1,2, WEI Zi-bin1,2
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1.Tianjin University of Traditional Chinese Medicine Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin 300193, China;2.Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin 300193, China
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| Abstract: |
| [Objective] To investigate ratintestinal absorption of Atractylenolide Ⅱ and Atractylenolide Ⅲ in Zexie decoction.[Methods] The column was Agilent Zorbax Plus C18 (4.6 mm×12.5 mm, 5 μm). The mobile phase consisted of a mixture acetonitrile-0.1% formic acid aqueous solution (42:58), the flow rate was 0.3 mL/min. Quantification was performed in the positive ionization by MRM mode. The monitored transitions were set at m/z 233.2→m/z187 and m/z 249.15→m/z231for Atractylenolide Ⅱ and Atractylenolide Ⅲ, respectively.[Results] The apparent permeability coefficients (Papp) of Atractylenolide Ⅱ was gradually on the increase in duodenum, jejunum, ileum and colon. The same thing happened to the Papp of Atractylenolidein Ⅲ in jejunum, duodenum, ileum and colon.[Conclusion] This method was simple, precise and repeatable, which could be used for intestinal investigation of Zexie decoction. |
| Key words: Zexie decoction Ussing chamber LC-MS/MS Atractylenolide Ⅱ Atractylenolide Ⅲ |
