甘草对H2O2诱导大鼠H9c2心肌细胞损伤保护作用的研究

Study on the effects of licorice on H2O2-induced H9c2 myocardial injury

关键词:  甘草  H₂O₂  H9c2  凋亡  氧化应激

Key words:  licorice  H₂O₂  H9c2  apoptosis  oxidative stress

摘要:

[目的]探讨甘草对双氧水（H₂O₂）诱导大鼠H9c2心肌细胞损伤的保护作用。[方法]建立H₂O₂诱导心肌细胞损伤模型（100 μmol/L，4 h），甘草（800、400 μg/mL）作用4 h，检测心肌细胞活力、乳酸脱氢酶（LDH）释放量、超氧化物歧化酶（SOD）和丙二醛（MDA）含量、细胞内氧自由基（ROS）含量、钙离子含量、线粒体膜通透性转换孔（mPTP）的开放及心肌细胞凋亡率。[结果]甘草能够不同程度提高心肌细胞活力和SOD活力，减少LDH、MDA、细胞内ROS和钙离子含量，抑制mPTP开放、降低细胞凋亡率（P<0.05或P<0.01）。[结论]甘草对H₂O₂诱导的大鼠H9c2心肌细胞损伤具有保护作用。

Abstract:

[Objective] To study the effects of licorice on H₂O₂-induced H9c2 myocardial injury.[Methods] H9c2 cells were treated with H₂O₂ (100 μmol/L for 4 h) to establish a myocardial injury model, and then treated licorice on the concentration of 400 μg/mL and 800 μg/mL for 4 h. The levels of cell viability, lactic dehydrogenas (LDH), superoxide dismutase (SOD), malondialdehyde (MDA), intracellular reactive oxygen species (ROS), intracellular calcium ion concentration, mitochondrial permeability transition pore (mPTP) and apoptosis rate were measured.[Results] Licorice can improve cell vitality and activity of SOD in different degree, reduce the LDH, MDA, ROS and Ca²⁺ content, inhibit mPTP opening and apoptosis rate (P<0.05 or P<0.01).[Conclusion] Licorice has the protective effect on H₂O₂-induced H9c2 myocardial cells.