甘草根系呼吸代谢与生物量积累的相关性分析

Correlation between root respiratory metabolism and biomass accumulation in G. uralensis

关键词:  甘草  呼吸代谢  生化途径  生物量  灰色关联度

Key words:  Glycyrrhiza uralensis Fisch.  respiratory metabolism  biochemical pathway  biomass  gray correlation degree

摘要:

[目的]研究甘草根系呼吸代谢与生物量积累的关联关系，为探索生理生化反应与目标收获量的可能影响机制奠定研究基础。[方法]溶氧法测定甘草根系呼吸强度；通过抑制关键酶的活性，测定各个基础生化途径的呼吸强度；采用烘干称质量法测定甘草地上部分和地下部分的生物量；通过灰色关联分析方法考察甘草根系呼吸代谢与生物量的相关关系。[结果]甘草根系以EMP-TCA呼吸途径和PPP呼吸途径为主，两条途径共同作用，9月上旬甘草根系呼吸代谢变化较显著，以EMP呼吸途径占总呼吸的比例最小，PPP呼吸途径占的比例最大，TCA的变化总体较小。TCA途径的呼吸强度与甘草地上部分生物量积累的关联度较大，关联系数为0.828 2，其次为PPP途径，而EMP途径的关联性较小（0.500）。3个基础生化途径与地下部分生物量积累均具有一定的关联度，但关联系数较小，且他们之间的差别不大。[结论]三羧酸循环、磷酸戊糖途径与甘草地上部分生物量积累的关联度较大，但3个基础生化途径与地下部分生物量积累的关联度差异不大。

Abstract:

[Objective] To study the correlation between root respiratory metabolism and biomass accumulation in G. uralensis. It would lay a foundation for the research on exploring the possible mechanism of physiological and biochemical reaction and target harvest.[Methods] The root respiratory intensity was determined using dissolved oxygen method. The basic biochemical pathways of root respiratory were measured by inhibiting activities of key enzymes. Index of aboveground and underground biomass were obtained by the oven drying method. And the correlation between root respiratory metabolism and biomass in G. uralensis was studied by gray relational analysis.[Results] EMP-TCA and PPP respiratory pathways were the main pathways in G.uralensis roots. The root respiratory metabolic changed more significant in mid-September, and the percentage of EMP respiratory pathway was smallest, while PPP respiratory pathway was highest, and TCA respiratory pathway changed not significant. Three basic biochemical pathways were ranked according to their gray correlation degree to aboveground biomass in G. uralensis as follows:TCA (0.828 2), PPP, EMP (0.500). Three basic biochemical pathways and underground biomass accumulation have a certain degree of correlation, but the correlation coefficient and the difference between them was small.[Conclusion] TCA and PPP have a great gray correlation degree with aboveground biomass accumulation in G. uralensis. There was no significant difference in the correlation between the three basic biochemical pathways and the underground biomass accumulation.