黄芩苷对LPS致流产小鼠的子宫局部免疫调节机制研究

Mechanism research of Baicalin regulating the local immunomodulatory mechanism of uterus by LPS induced abortion mice

关键词:  黄芩苷  妊娠  子宫  免疫调节  细胞因子  安胎

Key words:  baicalin  pregnancy  uterine  immune regulation  cytokine  tocolysis

摘要:

[目的]研究黄芩苷在子宫局部通过免疫调节发挥安胎作用机制，为黄芩"清热安胎"提供理论依据。[方法]将SPF级受孕鼠随机分为空白组（A组）、脂多糖（LPS）处理组（B组）、LPS及黄芩苷处理组（C组）和黄芩苷处理组（D组），分别比较四组的流产率、胚胎吸收率及通过ELSIA法测定的子宫组织中肿瘤坏死因子-α（TNF-α）和白介素（IL）-10因子含量的差异。[结果]1）流产率：B > A=C > D；2）胚胎吸收率：B > A≈C > D；3）TNF-α因子含量：B > C > A > D；4）IL-10因子含量：D > C > A > B；5）TNF-α/IL-10（即Th1/Th2）：B > A > C > D，且各组差异分别具有统计学意义（P<0.01）。[结论]黄芩苷在LPS致流产小鼠子宫局部免疫调节中，不仅可上调Th2型细胞分泌IL-10因子，使其含量明显升高，同时在抑制Th1型细胞，使其产生TNF-α因子数量显著减少，综合作用避免胚胎丢失，具有良好的安胎效果。

Abstract:

[Objective] To study the effects of Baicalin in the uterus through local immune modulation mechanism of tocolysis, provide a theoretical basis for "Scutellaria qingreantai".[Methods] the SPF pregnant rats were randomly divided into blank group (A) and LPS treatment group (B), LPS and baicalin treatment group (C) and baicalin treatment group (D), the differences were compared between the four groups of the abortion rate, embryo resorption rate and by ELSIA method for the determination of the uterus organization of TNF-alpha and IL-10 factor content.[Results] the abortion rate:B > A=C > D; the embryo resorption rate:B > A≈C > D;the TNF-alpha factor content:B > C > A > D; the IL-10 factor content:D > C > A > B; the TNF-alpha/IL-10 (Th1/Th2):B > A > C > D, and the differences were statistically signifycant(P<0.01).[Conclusion] baicalin in LPS induced local immune regulation in mouse uterus abortion, not only can secrete IL-10, upregulation of Th2 cells, which were significantly increased, while the inhibition of Th1 cells, which produce TNF-alpha factor significantly reduced quantity, avoid the comprehensive effect of embryo loss, has good effect of tocolysis.