羟基红花黄色素对博莱霉素致间质性肺病小鼠的作用机制研究

Study on the mechanism of Hydroxy safflower yellow pigment in bleomycin induced interstitial lung disease in mice

关键词:  羟基红花黄色素  间质性肺病  TGF-β1  IL-6  PAI-1  VEGF mRNA

Key words:  Hydroxy safflower yellow pigment  interstitial lung disease  TGF-β1  IL-6  PAI-1  VEGF mRNA

摘要:

[目的] 研究羟基红花黄色素对博莱霉素致间质性肺病小鼠的作用机制。[方法] 将90只KM小鼠随机分为对照组、模型组、红花黄色素组3组，建立雄性间质性肺病小鼠模型。实验第21天、第28天取右下肺分别做病理切片；第28天取左下肺叶用酶联免疫吸附测定（ELISA）法测定转化生长因子（TGF-β1）、白介素（IL）-6；第28天取右中上肺叶应用实时定量荧光PCR（RT-PCR）法检测血管内皮生长因子（VEGF）和内皮细胞型纤溶酶原激活物抑制因子（PAI-1）的表达。[结果] 与空白组比较，模型组小鼠肺组织TGF-β1、IL-6、PAI-1、VEGF mRNA表达水平明显升高（P<0.01）；与模型组比较，红花黄色素组小鼠肺组织TGF-β1含量明显降低（P<0.01），IL-6含量降低（P<0.05），红花黄色素组小鼠肺组织VEGF mRNA表达水平明显降低（P<0.01），PAI-1mRNA表达水平无差异。[结论] 羟基红花黄色素能降低TGF-β1、IL-6、VEGF mRNA表达水平，改善肺纤维化。

Abstract:

[Objective] To study the mechanism of Hydroxy safflower yellow pigment in bleomycin induced interstitial lung disease in mice.[Methods] A total 90 Balb/c mice were divided into 3 groups, that is control group, model group group and Hydroxy safflower yellow pigment group. After 21days, 3 mice were dissected at random,and After 28 days,all mice were dissected and lung tissues were taken,while the experiment was finished. Masson staining can show the degree of inflammatory cell infiltration in mice lung tissues; Enzyme-linked immunosorbent assay was used to detect the expression levels of TGF-β1, IL-6; RT-PCR assay was applied to detect the expression levels of PAI-1and VEGF in protein levels.[Results] The expression of TGF-β1、VEGF mRNA in model group were higher significantly than control group(P<0.01), while these indexes of Hydroxy safflower yellow pigment group lower significantly thanmodel group(P<0.01). The expression of IL-6 in model group were higher significantly than control group(P<0.01), while this indexes of Hydroxy safflower yellow pigment group lower thanmodel group(P<0.05). The expression of PAI-1 mRNA in model group were higher significantly than control group(P<0.01), while this indexes of Hydroxy safflower yellow pigment group was not significantly correlated.[Conclusion] Hydroxy safflower yellow pigment can reduce the expression of TGF-β1, IL-6, VEGF mRNA, and prevent or reverse pulmonary fibrosis.