旋覆代赭汤对胆碱能抗炎通路相关RE大鼠炎症因子的影响

The effect of Xuanfu Daizhe decoction on inflammations in Reflux Esophagitis rats of CAP

关键词:  旋覆代赭汤  胆碱能抗炎通路  反流性食管炎  炎症因子

Key words:  Xuanfu daizhe decoction  CAP  reflux esophagitis  inflammations

摘要:

[目的] 通过观察旋覆代赭汤对胆碱能抗炎通路相关反流性食管炎（RE）大鼠食管组织和血浆中肿瘤坏死因子（TNF）-α，白介素（IL）-1β，IL-6和IL-10含量影响，探讨旋覆代赭汤对反流性食管炎的保护机制。[方法] 将大鼠随机分为正常组、假手术组、RE组、模型组、中药组和西药组。用“颈左侧迷走神经切断术”联合“贲门肌切开术加部分幽门结扎”制备大鼠反流性食管炎动物模型，造模3周后，各组给予相应药物连续灌胃治疗2周。观察各组大鼠食管肉眼和组织学变化，用酶联免疫吸附剂测定（ELISA）法检测食管组织和血浆中TNF-α，IL-1β，IL-6和IL-10含量。[结果] 中药组大鼠食管黏膜炎症较模型组明显改善，组织中TNF-α，IL-1β，IL-6含量较模型组均降低（P<0.05或P<0.01），血浆中TNF-α，IL-1β含量较模型组无统计学差异。中药组与模型组比较，IL-10含量无统计学差异。[结论] 旋覆代赭汤可以改善RE大鼠食管黏膜炎症，可以降低食管组织中TNF-α，IL-1β，IL-6含量，对IL-10无影响。

Abstract:

[Objective] Observed the content of TNF-α, IL-1β, IL-6 and IL-10 in esophageal tissue and plasma of reflux esophagitis (RE) rats, to explore the protection mechanism of Xuanfu daizhe decoction.[Methods] Rats were randomly divided into normal group, the RE group, control group, model group, Chinese medicine and western medicine group. The Model group were administered "left cervical vagotomy" and "cardiomyotomy combine with pylorus ligation". After 3 weeks, each rats were treated with the corresponding drug for 2 weeks, the rats were sacrifice, collected the esophageal tissue and plasma. Observed the pathological morphological changes of each rats esophageal tissue.Using ELISA method to detect TNF-α, IL-1β, IL-6 and IL-10 levels in esophageal tissue and plasma.[Results] The esophageal mucosal inflammation in Chinese medicine group obviously improved compared with model group, the content of TNF-α, IL-1 beta, IL-6 in Chinese medicine group were lower than the model group (P<0.05 or P<0.01), the content of TNF-α, IL-1β in plasma, compared with model group, there was no significant difference (P>0.05). The content of IL-10 in Chinese medicine group compared with model group, there was no statistical difference.[Conclusion] Xuanfu daizhe decoction can improve RE rats' esophageal mucosal inflammation, by decreasing the content of TNF-α, IL-1β and IL-6 in esophageal tissue, but have no effect on IL-10 levels.