银盏心脉滴丸对缺氧/复氧损伤大鼠心肌细胞线粒体功能的影响

Effect of Yinzhan Xinmai dropping pill on mitochondrial function of myocardial cells in rats with hypoxia/reoxygenation injury

关键词:  银盏心脉滴丸  线粒体功能  细胞凋亡

Key words:  Yinzhan Xinmai dropping pill  mitochondrial function  apoptosis

摘要:

[目的]探讨银盏心脉滴丸（YZXM）含药血清对缺氧/复氧损伤大鼠心肌细胞线粒体功能的影响。[方法]以大鼠心室肌细胞（H9C2）为研究对象，制备银盏心脉滴丸含药血清，分对照组、模型组（缺氧/复氧损伤）、YZXM组（30 min、12 h和24 h），采用流式细胞术检测细胞凋亡率，采用海马能量测定仪测量细胞基础呼吸、最大呼吸率、储备能力、ATP生成量。[结果]与对照组相比，模型组细胞凋亡指数高于对照组（P<0.01）。与模型组相比，YZXM组细胞凋亡指数降低（P<0.01）。YZXM增加缺氧/复氧损伤的H9c2细胞线粒体基础耗氧率、ATP生成量、最大呼吸以及储备能力（n=4，P<0.05）。[结论]银盏心脉滴丸通过改善线粒体功能及能量代谢减轻细胞凋亡保护缺氧/复氧心肌细胞。

Abstract:

[Objective] To investigate the effect of Yinzhan Xinmai dropping pill (YZXM) on mitochondrial function of myocardial cells in rats with hypoxia/reoxygenation injury.[Methods] H9C2 cardiomyocytes were divided into 5 groups. The control group, model group (anoxic/reoxygenation injury), YZXM group (30 min, 12 h and 24 h). Flow cytometry was used to detect apoptotic cardiomyocytes. The XF-96 extracellular flux analyzer was used to measure basic breath function, the maximum respiratory rate, the level of ATP, and respiratory ctrl ratios of H9c2.[Results] Compared with the control group, the apoptosis index of the model group was higher than that of the control group (P<0.01). Compared with the model group, the apoptosis index of the YZXM group was decreased (P<0.01). YZXM increased mitochondrial basal oxygen consumption rate, ATP-related oxygen consumption rate, maximum oxygen consumption rate and reserve capacity of H9c2 cells in hypoxia/reoxygenation injury (n=4, P<0.05).[Conclusion] Yinzhan Xinmai drop pill protects hypoxia/reoxygenation cardiomyocytes by improving mitochondrial function and energy metabolism to reduce apoptosis.