通络止痛方对不同程度膝骨关节炎组织形态学和固有免疫的影响

The effect of Tongluo zhitong formulation on TLRs/MyD88 signaling pathway of knee osteoarthritis in different degrees

关键词:  TLRs/MyD88信号通路  通络止痛方  膝骨关节炎  滑膜炎症  作用机制

Key words:  TLRs/MyD88 signal pathway  Tongluo Zhitong formulation  knee osteoarthritis  synovial inflammation  mechanism of action

摘要:

[目的]评估通络止痛方对不同程度膝骨关节炎滑膜和软骨的组织形态学及固有免疫的影响。[方法]利用经典Hulth法建立轻度、中度、重度大鼠KOA动物模型，运用超声促透通络止痛方的方法对大鼠KOA模型进行干预，通过观察滑膜及软骨形态学变化确定病变程度，并对软骨进行Mankin's评分。利用免疫荧光双标技术，观察软骨和滑膜上参与结构破坏的主要蛋白TLR4、NF-κB、MyD88表达量的变化。[结果]第2、4、8周组各组内通络止痛方干预组滑膜和软骨损伤程度较对照组明显减轻。在固有免疫中，各组内通络止痛方干预组的TLR4、NF-κB、MyD88的表达较对照组减弱。[结论]通络止痛方对不同程度膝骨关节炎均有抑制作用，证明通络止痛方可以通过抑制TLRs/MyD88信号通路相关原件的表达，抑制膝骨关节炎的发生和发展。

Abstract:

[Objective] To evaluate the effect of Tongluo Zhitong formulation on histomorphology and innate immunity of synovium and cartilage in knee osteoarthritis of different degrees.[Methods] The KOA model of rats was established by Hulth method. The KOA model of rats was intervened by the method of ultrasound promoting Tongluozhitong formulation. The pathological changes of synovium and cartilage were observed to determine the degree of lesion, and the cartilage was evaluated by Mankin's score. The expression of TLR4, NF-κB and MyD88 in cartilage and synovium were observed by immunofluorescence double labeling technique.[Results] The injury degree of synovium and cartilage in the intervention group of Tongluo Zhitong formulation at 2, 4 and 8 weeks was significantly less than that in the control group. In innate immunity, the expression of TLR4, NF-κB and MyD88 in the intervention group of Tongluo Zhitong formulation was weaker than that in the control group.[Conclusion] Tongluo Zhitong formulation can inhibit knee osteoarthritis in different degrees, which proves that Tongluo Zhitong formulation can inhibit the occurrence and development of knee osteoarthritis by inhibiting the expression of TLRs/MyD88 signaling pathway related components.