清热利湿方对脂肪肝细胞PI3K/Ark表达调节研究

Qingre Lishi Recipe regulate of PI3K/Ark expression in fatty liver cells

关键词:  脂肪肝  中药  PI3K  Ark

Key words:  fatty liver  TCM  PI3K  Ark

摘要:

[目的] 观察清热利湿方对脂肪肝细胞酸肌醇3-激酶（PI3K/Ark）通路调节作用。[方法] 选取大鼠60只，随机数字表法将大鼠分为6组：正常对照组、高脂模型组、多烯磷脂酰胆碱组、清热高剂量组、清热中剂量组、清热低剂量组，采用高脂饲料复制大鼠脂肪肝实验动物模型，6周开始清热利湿组分别给与给予0.3 g/（100 g·d）（清热高剂量组）0.1 g/（100 g·d）（清热中剂量组），0.05 g/（100 g·d）（清热低剂量组）灌胃，多烯组给予25 mg/（100 g·d）灌胃，12周后取材。病理检查各组脂肪肝程度，ELISA检测血清急性时相反应蛋白（hs-CRP）含量及ATP酶活性，生化分析仪检测脂肪肝各实验组血脂（TC/TG）、肝功能（ALT/AST/GGT）。Western-blot检测脂肪变肝细胞磷脂酰肌醇-3激酶（PI3K）/Ark表达。[结果] 高脂模型组hs-CRP明显升高，与清热高剂量组比较具有明显差异（P<0.05）。各实验组ATPase呈现不同程度降低，清热高剂量组血清ATPase水平与高脂模型组比较具有明显差异（P<0.05）；Western blot实验结果可见，各实验组大鼠肝细胞PI3K/Ark表达存在明显差异，清热利湿方治疗后，明显降低表达PI3K/Ark水平。[结论] 清热利湿方通过调节ATPase、hs-CRP、PI3K/Ark肝细胞氧化应激炎症介质，改善肝细胞由于脂类代谢异常形成的过氧化损伤，促进肝细胞功能的恢复，具备一定的改善脂肪变性效果。

Abstract:

[Objective] To observe the regulation effect of Qingre Lishi Recipe on acid inositol 3-kinase (PI3K) pathway in fatty liver rats.[Methods] A total of 60 rats were randomly divided into 6 groups:normal control group,high fat model group,polyene phosphatidylcholine group,Qingre Lishi Recipe high dose group,Qingre Lishi Recipe medium dose group,and Qingre Lishi Recipe low dose group. The animal model of rat fatty liver was replicated by high-fat diet,and the heat-clearing and dampness group was intragastrically given 0.3 g/(100 g·d) (Qingre Lishi high-dose group),0.1 g/(100 g·d)(Qingre Lishi medium-dose group) and 0.05 g/(100 g·d)(Qingre Lishi low-dose group),and the polyene group was given 25 mg/(100 g·d). After 12 weeks,the material was taken. Pathological examined the degree of fatty liver in each group,by ELISA detected serum acute phase reaction protein (hs-CRP) content and ATPase activity,in biochemical analyzer detected fatty liver test group blood lipids (TC/TG),liver function (ALT/AST/GGT). Western-blot was used to detect phosphatidylinositol 3-kinase (PI3K)/Ark expression in fatty liver cells.[Results] The hs-CRP level in the high-fat model group was significantly higher than that in the high-dose group (P<0.05). The ATPase levels in each experimental group were decreased to different extents. The serum ATPase levels in the high-dose group were significantly different from those in the high-fat model group (P<0.05). The results of western-blot showed that the expression of PI3K/Ark in the hepatocytes of each experimental group existed significant differences,reduced expression of PI3K/Ark after treatment with Qingre Lishi recipe.[Conclusion] Qingre Lishi Recipe can improve the hepatocyte function recovery by improving the oxidative stress inflammatory mediators of ATPase,hs-CRP and PI3K/Ark hepatocytes,and can promote the recovery of liver cell function,at the same time,it also has certain effect of improving fatty degeneration.