| 摘要: |
| [目的] 建立重黄温敏凝胶中重楼皂苷VI的含量测定方法，并进行体外释放研究。[方法] 采用HPLC-ELSD法，色谱柱为CAPCELL PAK C8柱 （4.6 mm×250 mm，5μm），乙腈（A）-水（B）为流动相，梯度洗脱（0~15 min，25%~35% A；15~35 min，35%~40% A；35~40 min，40%~60% A），柱温25℃，流速1.0 mL/min，进样量10 μL，漂移管温度92℃，载气流速2.9 L/min。以体外溶蚀法，对重黄温敏凝胶的累积释放情况，进行考察。[结果] 重楼皂苷VI在0.430 0~4.300 0 mg范围内线性良好，lgA=1.796 9lgC +4.398 9（r2=0.999 9），定量限为0.054 0 mg，平均回收率为101.70%（RSD=1.17%，n=9），体外释放符合Higuchi方程。[结论] 该方法方便、稳定、高效，可用于重黄温敏凝胶体外释放研究。 |
| 关键词: 重黄温敏凝胶 重楼皂苷VI 体外释放 高效液相色谱-蒸发光散射检测 |
| DOI：10.11656/j.issn.1673-9043.2020.02.23 |
| 分类号:R284.1 |
| 基金项目:云南省科技厅项目（2018DC002）。 |
|
| Determination and release in vitro of polyphyllin VI in ChongHuang thermosensitive in situ gel |
|
SUN Yuan1,2,3, DING Jiangsheng1,2,3, WAN Jinfu1,2,3
|
|
1.Yunnan Institute of Materia Medica, Kunming 650111, China;2.Yunnan Baiyao Group Innovation and R&D Center, Kunming 650111, China;3.Yunnan Province Company Key Laboratory for Traditional Chinese Medicine and Ethnic Drug of New Drug Creation, Kunming 650111, China
|
| Abstract: |
| [Objective] To establish a determination method for the content of polyphyllin VI in ChongHuang thermosensitive in situ gel and to study the release in vitro. [Methods] A HPLC-ELSD method was adopeted with the CAPCELL PAK C8 column (4.6 mm×250 mm,5 μm),with the mobile phaseconsisting of acetonitrile(A)-water(B)at the flow rate of 1.0 mL/min (0~15 min,25%~35% A;15~35 min,35%~40%A;35~40 min,40%~60%A),with the column temperature 25℃,with the injection volume 10 μL,with the drift tube temperature 92℃ and the carrier gas pressure was 2.9 L/min. [Results] The polyphyllin VI has good linearity in the range of 0.430 0~4.300 0 mg,lgA=1.796 9lgC+4.398 9(R2=0.999 9),The average recovery rate was 101.70% (RSD=1.17%,n=9). The in vitro release study indicated that drug release of polyphyllin VI was best fitted to Higuchi equation. [Conclusion] The established method is proved to be convenient,stable and efficient. |
| Key words: Chonghuang thermosensitive in situ gel polyphyllin VI in vitro release HPLC-ELSD |
