基于UPLC-Q-TOF/MS技术的橘皮竹茹汤血清药物化学初步研究

Preliminary study on serum pharmacochemistry of Jupi Zhuru Decoction based on UPLC-Q-TOF/MS

关键词:  橘皮竹茹汤  血清药物化学  超高效液相色谱-四级杆-飞行时间串联质谱联用  柚皮黄素  刺芒柄花素

Key words:  Jupi Zhuru Decoction  serum pharmacochemistry  UPLC-Q-TOF/MS  natsudaidain  formononetin

摘要:

[目的] 对橘皮竹茹汤进行血清药物化学初步研究。[方法] 大鼠灌胃橘皮竹茹汤冻干粉，采集含药血清。采用超高效液相色谱-四级杆-飞行时间串联质谱联用（UPLC-Q-TOF/MS）技术，对橘皮竹茹汤冻干粉样品、空白血清及给药血清图谱进行比较，并利用Peakview和MetabolitePilot数据处理软件，根据质谱所提供的保留时间、精确相对分子质量及二级质谱裂解碎片来鉴定和推测其血中移行成分。[结果] 橘皮竹茹汤冻干粉灌胃给药后，从血清中检测出41个入血成分，其中原型成分13个、代谢产物28个。[结论] 初步确定了橘皮竹茹汤的入血成分，为阐明其药效物质基础提供了依据。

Abstract:

[Objective] To study the serum pharmacochemistry of Jupi Zhuru Decoction.[Methods] The rats were given the freeze-dried powder of Jupi Zhuru Decoction by gavage, and the serum containing the drug was collected. Ultra high performance liquid chromatography quadrupole time of flight mass spectrometry(UPLC-Q-TOF/MS) was used to compare the chromatograms of Jupi Zhuru Decoction powder, blank serum and administered serum. The data processing software of MetabolitePilot and Peakview were used to identify and infer the migration components in blood according to the retention time, accurate relative molecular mass and fragmentation fragments of secondary mass spectrometry.[Results] After intragastric administration of Jupi Zhuru Decoction lyophilized powder, total of 41 blood components were detected in serum, of which 13 were prototype components and 28 were metabolites.[Conclusion] The blood components of Jupi Zhuru Decoction are preliminarily determined, which provides the basis for elucidating its pharmacodynamic material basis.