补骨脂素、异补骨脂素和补骨脂酚急性毒性的性别差异研究

An acute toxicity study of psoralen,isopsoralen and bakuchiol in different gender

关键词:  补骨脂素  异补骨脂素  补骨脂酚  急性毒性  性别差异

Key words:  psoralen  isopsoralen  bakuchiol  acute toxicity  gender differences

摘要:

[目的] 探究补骨脂素、异补骨脂素和补骨脂酚对小鼠急性毒性的性别差异。[方法] 补骨脂素给药剂量为雌性（1 125、843、633、475、356 mg/kg）、雄性（633、475、356、267、200 mg/kg），异补骨脂素给药剂量为雌性（404、343、292、248、210 mg/kg）、雄性（317、292、269、248、229 mg/kg），补骨脂酚雌、雄剂量均为2 000、1 765、1 500、1 200、1 020 mg/kg。一次性灌胃给予受试药，连续观察并记录14 d小鼠的毒性反应、体质量和死亡情况，应用SPSS统计学软件，采用Probit回归法计算半数致死量（LD₅₀）和95%可信区间。[结果] 给药后小鼠毒性反应主要表现为抽搐、四肢强直，部分动物口眼周围有分泌物，剂量越高反应越明显。与对照组比较，补骨脂素、异补骨脂素给药组随给药剂量增大，小鼠体质量呈现降低趋势。补骨脂素雌性小鼠的LD₅₀为514.35mg/kg，雄性小鼠LD₅₀为421.37 mg/kg，雌性小鼠LD₅₀值比雄性高出22%。异补骨脂素雌性小鼠LD₅₀为258.02 mg/kg，雄性小鼠LD₅₀为255.26 mg/kg。补骨脂酚雌性小鼠LD₅₀超出2 000 mg/kg，雄性小鼠LD₅₀为1 679.4 mg/kg。[结论] 补骨脂素和补骨脂酚对小鼠的急性毒性存在一定性别差异，但无统计学意义。

Abstract:

[Objective] To investigate the gender differences in acute toxicity of psoralen, isopsoralen and bakuchiol in mice.[Methods] The doses of psoralen were female (1 125, 843, 633, 475, 356 mg/kg) and male (633, 475, 356, 267, 200 mg/kg). For isopsoralen, the doses were female (404, 343, 292, 248, 210 mg/kg), male (317, 292, 269, 248, 229 mg/kg) and the doses of psoralen were 2 000, 1 765, 1 500, 1 200, 1 020 mg/kg for both. The test drugs were administered by intragastric administration, and the toxic effects, body mass and death of the mice were observed and recorded for 14 days. SPSS statistical software was used to calculate the LD₅₀ and 95% confidence limit of the median lethal dose by Probit regression.[Results] The toxicity of the mice after administration was mainly convulsions, limbs and straightness, and the abdomen was attached to the ground. Some animals had secretions around the eyes. The higher the dose, the more obvious the reaction. Compared with the control group, the body weight of the mice in the psoralen and isopsoralen administration groups decreased with increasing dose. The LD₅₀ of psoralen female mice was 514.35 mg/kg;the LD₅₀ of male mice was 421.37 mg/kg, and the LD₅₀ value of female mice was 22% higher than that of males. The LD₅₀ of female mice with isopsoralen was 258.02 mg/kg;the LD₅₀ of male mice was 255.26 mg/kg. The LD₅₀ of bakuchiol female mice exceeded 2 000 mg/kg;the LD₅₀ of male mice was 1 679.4 mg/kg.[Conclusion] There is a gender difference in the acute toxicity of psoralen and bakuchiol in mice, but without statistical significance.