扶正胶囊提取物对环磷酰胺诱导的免疫低下小鼠免疫调节作用研究

Study on immunomodulatory effect of Fuzheng Capsule extract on immunocompromised mice induced by cyclophosphamide

关键词:  扶正胶囊提取物  环磷酰胺  免疫抑制  淋巴细胞亚群

Key words:  Fuzheng Capsule extract  cyclophosphamide  immunosuppression  lymphocytesubpopulation

摘要:

[目的] 探讨扶正胶囊提取物对免疫抑制小鼠免疫功能的调节作用。[方法] 将60只昆明小鼠进行随机分组，每组10只，分为空白组、模型组、阳性对照组以及扶正胶囊提取物低、中、高剂量组。采用环磷酰胺腹腔注射建立小鼠免疫功能低下模型，检测各组小鼠的胸腺指数和脾脏指数、单核细胞吞噬能力、血清中白细胞介素-4（IL-4）、白细胞介素10（IL-10）、白细胞介素-17（IL-17）、肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）水平和脾T淋巴细胞亚群CD3⁺T淋巴细胞（CD3⁺）、CD4⁺T淋巴细胞（CD4⁺）、CD8⁺T淋巴细胞（CD8⁺）的分布与比例。[结果] 扶正胶囊提取物能提升由环磷酰胺诱导的免疫低下小鼠的胸腺指数和脾脏指数，增强单核细胞吞噬功能，显著提高小鼠细胞免疫因子IL-4、IL-10、IFN-γ含量，降低IL-17、TNF-α含量，上调脾淋巴细胞亚群CD3⁺CD4⁺的表达程度和提升CD3⁺CD4⁺/CD3⁺CD8⁺比值。[结论] 扶正胶囊提取物能够提升环磷酰胺所致的免疫抑制小鼠的免疫功能。

Abstract:

[Objective] To investigate the regulation effect of Fuzheng Capsule extract on immune function of immunosuppressed mice.[Methods] Sixty Kunming mice were randomly divided into blank group, model group, positive control group, and Fuzheng Capsule extract low-dose, medium-dose and high-dose groups with 10 mice in each group.The immunocompromise model of mice was established by intraperitoneal injection of cyclophosphamide. Thymus index and spleen index, monocyte phagocytic, serum levels of Interleukin 4 (IL-4), Interleukin 10 (IL-10), Interleukin 17 (IL-17), Tumor necrosis factor alpha (TNF-α), Interferon gamma (IFN-γ) and the distribution and proportion of CD3⁺T lymphocytes(CD3⁺), CD4⁺T lymphocytes (CD4⁺)、CD8⁺T lymphocytes (CD8⁺).[Results] Fuzheng capsule extract can improve the thymus index and spleen index of immunosuppressed mice induced by cyclophosphamide, enhance the phagocytic function of monocytes, significantly increase the contents of cytokines IL-4, IL-10, IFN-γ, and decrease the contents of IL-17, TNF-α.The expression of CD3⁺CD4⁺ and the ratio of CD3⁺CD4⁺/CD3⁺CD8⁺ were up-regulated.[Conclusion] Fuzheng Capsule extract can improve the immune function of immunosuppressed mice induced by cyclophosphamide.