| 摘要: |
| [目的] 探索自拟方甘草养阴汤（GNY）对咪喹莫特（IMQ）诱导的银屑病样皮肤损伤小鼠模型的疗效。[方法] 将小鼠分为健康对照组、模型组（IMQ诱导银屑病小鼠）和治疗组（给予银屑病小鼠GNY，浓度为2.5、5 g/kg）；共4组（动物数=8/组）。采用银屑病病灶面积和严重程度指数（PASI）、苏木-精伊红染色和Baker评分用于评估疾病的严重程度。采用免疫组织化学或免疫荧光染色法对真皮的Gr-1，CD11c，RORγt和TGF-β阳性的炎性细胞进行计数。采用流式细胞仪分析脾脏细胞中的CD4+IL-17+Th17细胞和CD4+CD25+Foxp3+Treg细胞比例。使用qPCR半定量IL-6、IL-10、IL-17A、IL-22、IL-23、TNF-α、TGF-β和AMPK-1的相对mRNA表达。[结果] 与模型组相比，GNY治疗的小鼠在两种浓度下均能够改善了病灶的形态学特征，包括PASI评分和上皮角质细胞增生。较高浓度的GNY可显着降低银屑病小鼠模型真皮中的Gr-1，CD11c，RORγt和TGF-β阳性细胞比例；下调脾脏Th17细胞比例，增加Treg细胞的表达。此外，IMQ模型组小鼠的真皮内IL-6、IL-10、IL-17A、IL-22、IL-23、TNF-α、TGF-β和AMPKɑ1的相对mRNA表达升高，在以5g/kg浓度的GNY治疗的模型小鼠中表达明显降低。[结论] 研究表明GNY可改善IMQ诱导的小鼠银屑病样皮肤损伤表现、减轻炎症反应；为临床治疗银屑病提供依据。 |
| 关键词: 动物模型 咪喹莫特 调节性T细胞/Th17细胞 银屑病 中药 |
| DOI：10.11656/j.issn.1673-9043.2022.04.12 |
| 分类号:R275.9 |
| 基金项目:广东省中医药局科研项目（20201229） |
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| Effect of Gancao Yangyin Decoctionon improving psoriasis-like skin lesions and regulating inflammation in mice |
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WU Huimei1, CHEN Yong2,3, XIAO Changhong3, NANDAKUMAR Kutty Selva1
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1.Southern Medical Univerisity-Karolinska Institute United Medial Inflammation Center, College of Pharmacy, Southern Medical University, Guangzhou 510515, China;2.Department of Rheumatology and Immunology, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen 518020, China;3.Department of Rheumatology, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510315, China
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| Abstract: |
| [Objective] To explore the efficacy of self-made Gancao Yangyin Decoction (GNY) on imiquimod (IMQ)-induced psoriasis-like inflammation.[Methods] The mice were divided into healthy control group, model group (IMQ-induced psoriasis mice) and treatment group(GNY concentration of 2.5 g/kg and 5 g/kg given to psoriasis mice. A total of 4 groups, each group has 8 mice. Psoriasis area and severity index (PASI), HE staining and Baker's scores of skin were used to assess the severity of the disease. Immunohistochemistry or immunofluorescence staining was used for detection of Gr-1, CD11c, RORγt and TGF-β in the skin. Flow cytometry was used to analyze Th17 cells and Treg cells expression in spleen. The relative mRNA expression of IL-6, IL-10, IL-17A, IL-22, IL-23, TNF-α, TGF-β and AMPK-1 were examined by qPCR.[Results] Compared with the model group, psoriasis treated by GNY are improve at both concentrations. The morphological characteristics of the psoriasis lesions include increasesd PASI scores and epidermis thickness, which can be reduced after GNY treatment; higher concentrations of GNY significantly reduced Gr-1, CD11c, RORγt and TGF-β positive cells expression in skin; Down-regulation of Th17 cells but increase of Treg cells was found in the spleen after GNY treatment. In addition, the increase of IL-6, IL-10, IL-17A, IL-22, IL-23, TNF-α, TGF-β and AMPKɑ1 induced by IMQ were significantly reduced after treatment of GNY with the concentration of 5 g/kg.[Conclusion] This study proved that GNY improve the inflammation induced by IMQ, which provides a basis for clinical treatment of psoriasis. |
| Key words: animal model imiquimod poriasis Treg/Th17 traditional Chinese medicine |
