清脑益智方对血管性痴呆大鼠脑血流量及血脂水平的影响

Effect of Qingnao Yizhi Formulation on cerebral blood flow and blood lipid level in vascular dementia rats

关键词:  清脑益智方  血管性痴呆  脑血流量  血脂水平

Key words:  Qingnao Yizhi Formulation  vascular dementia  cerebral blood flow  blood lipid level

摘要:

[目的] 考察清脑益智方（QNYZF）对血管性痴呆（VaD）大鼠脑血流量（CBF）及血脂水平的影响。[方法] 选用健康雄性SD大鼠，随机分为假手术组、模型组、QNYZF低、中、高剂量组，采用双侧结扎颈总动脉（2-VO）法复制大鼠VaD模型。灌胃给药4周后，采用MOOR激光多普勒血流监测仪检测大鼠CBF，测完后取血、离心，收集血清，通过全自动生化仪检测血清中总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白胆固醇（LDL-C）和高密度脂蛋白胆固醇（HDL-C）含量。[结果] 与假手术组比较，模型组大鼠CBF降低（P<0.05）；与模型组比较，给予QNYZF干预后，中、高剂量组大鼠CBF升高（P<0.05或P<0.01）。与假手术组比较，模型组大鼠血清中TC、TG和LDL-C含量升高（P<0.05），而HDL-C含量无明显变化（P>0.05）；给予QNYZF干预后，各剂量的QNYZF均可降低VaD大鼠血清中TC、TG和LDL-C含量（P<0.05或P<0.01），而对HDL-C含量无明显影响（P>0.05）。[结论] QNYZF可有效改善VaD大鼠的CBF，其作用机制与降低血脂水平、改善血脂异常有关。

Abstract:

[Objective] To investigate the effect of Qingnao Yizhi formula(QNYZF) on cerebral blood flow and blood lipid level in vascular dementia (VaD) rats.[Methods] The healthy male SD rats were randomly divided into sham operation group, model group, low, middle and high doses of QNYZF groups. The rat VaD model was replicated by bilateral ligation of the common carotid artery(2-VO). After 4 weeks of intragastric administration, the rats' cerebral blood flow was measured with a Moor laser Doppler blood flow monitor. After the measurement, blood was taken and centrifugated. Serum was collected, and the content of total cholesterol(TC), triglyceride(TG), low-density lip-oprotein cholesterol(LDL-C) and high-density lipoprotein cholesterol(HDL-C) in serum was detected by an automatic biochemical analyzer.[Results] Compared with the sham operation group, the cerebral blood flow of rats in the model group was significantly reduced (P<0.05);compared with the model group, after the intervention of QNYZF, the cerebral blood flow of rats in the middle and high dose groups was significantly increased(P<0.05 or P<0.01). Compared with the sham operation group, the serum levels of TC, TG and LDL-C in the model group rats were significantly increased(P<0.05), while the level of HDL-C did not change significantly(P>0.05). After administration of QNYZF, the serum levels of TC, TG and LDL-C in VaD rats were significantly decreased(P<0.05 or P<0.01), but had no significant effect on HDL-C content(P>0.05).[Conclusion] QNYZF can effectively improve the cerebral blood flow of VaD rats through reducing the blood lipid level and improving the dyslipidemia.