老年性皮肤瘙痒症血虚风燥证动物模型构建及猪肤汤软膏的干预作用

Establishment of animal model of senile pruritus with blood deficiency and wind dryness syndrome and intervention effect of Zhufutang Ointment

关键词:  老年性皮肤瘙痒症  血虚风燥证  动物模型

Key words:  senile pruritus  blood deficiency and wind dryness syndrome  animal model

摘要:

基于“病证结合”理论，构建和评价老年性皮肤瘙痒症（SP）血虚风燥证小鼠模型。[方法] 研究以小鼠体质量为区组因素，采用随机区组设计，将72只C57BL/6小鼠随机分入空白组、模型组、地奈德乳膏组（阳性对照组）、猪肤汤软膏低浓度组、猪肤汤软膏中浓度组、猪肤汤软膏高浓度组，共6组，每组12只小鼠。采用先病后证的造模思路，除空白组外，其余5组采用D-半乳糖注射结合丙酮、乙醚混合液刺激法建立SP动物模型，再采用低铁饲料喂养结合物理刺激建立血虚风燥证模型。造模结束后，通过小鼠一般情况、表观指标、病理指标和生化指标来验证模型是否建立成功。[结果] 1）小鼠一般情况：造模前各组小鼠皮肤状况良好，眼神明亮，精神状况良好。与空白组相比，造模后其余各组小鼠出现烦躁不安、易激惹、大便干燥等现象（P<0.05）。2）表观指标评价：造模前各组小鼠搔抓行为和饮水量比较差异无统计学意义（P>0.05）；与空白组相比，造模后其余各组搔抓行为、饮水量均增加（P<0.05）。3）病理指标评价：干预后，与模型组相比，地奈德乳膏组（阳性对照组）、猪肤汤软膏低浓度组、猪肤汤软膏中浓度组、猪肤汤软膏高浓度组小鼠表皮厚度、皮肤肥大细胞计数均降低（P<0.05）。4）生化指标评价：干预后，与模型组相比，地奈德乳膏组（阳性对照组）、猪肤汤软膏低浓度组、猪肤汤软膏中浓度组、猪肤汤软膏高浓度组小鼠肿瘤坏死因子-α（TNF-α）水平降低（P<0.05），白细胞介素-4（IL-4）、羟脯氨酸（Hyp）水平升高（P<0.05）。[结论] 研究采用先病后证的造模思路，通过D-半乳糖注射结合丙酮、乙醚混合液刺激，采用低铁饲料喂养及物理刺激成功建立了SP血虚风燥证动物模型，该模型稳定性较好，操作性相对较强，值得进一步应用。

Abstract:

[Objective] Based on the theory of "combination of disease with syndrome", we established and evaluated the senile pruritus(SP) animal model of blood deficiency and wind-dryness.[Methods] In this study, taking body weight as a block factor, 72 C57BL/6 mice were randomly divided into 6 treatment groups, namely blank group, model group, desonide cream group, Zhufutang Ointment low concentration group, Zhufutang Ointment medium concentration group and Zhufutang Ointment high concentration group, with 12 mice in each group. Adopting the idea of making model of disease before syndrome, in addition to the normal group, the other 5 groups used D-galactose Injection combined with the mixture of acetone and ether stimulation method to establish SP animal model, and then the low iron feed feeding combined with physical stimulation can successfully establish the SP animal model of blood deficiency and wind dryness. After modeling, the success of the model was verified by the general condition, apparent indicators, pathological index and biochemical index of mice.[Results] 1)General situation of mice:before modeling, mice in each group had good skin condition, bright eyes and good mental condition. Compared with the blank group, mice in the other groups appeared estless, irritable and dry stool after modeling(P<0.05). 2)Evaluation of apparent indicators:there was no significant difference in scratching behavior and water intake between each group before modeling. Compared with the blank group, the scratching behavior and water intake of the other groups were increased after modeling(P<0.05). 3)Pathological evaluation:after intervention, compared with the model group, the epidermal thickness and mast cell count of mice in the desonide cream group, Zhufutang Ointment low concentration group, Zhufutang Ointment medium concentration group and Zhufutang Ointment high concentration group decreased(P<0.05). 4)Biochemical index evaluation:after intervention, compared with model group, the level of TNF-α in mice of the desonide cream group, Zhufutang Ointment low concentration group, Zhufutang Ointment medium concentration group and Zhufutang Ointment high concentration group decreased (P<0.05), IL-4 cytokine and HYP level increased (P<0.05).[Conclusion] In this study, the animal model of SP blood deficiency and wind dryness syndrome was successfully established by using the idea of disease and then syndrome, D-galactose Injection combined with acetone and ether mixture stimulation, low-iron feed feeding and physical stimulation. The model has good stability and relatively strong operability, and is worthy of further application.