气相色谱法快速测定水蛭中短链脂肪酸含量

Rapid determination of short chain fatty acids in leech by gas chromatography

关键词:  水蛭  短链脂肪酸  气相色谱

Key words:  leech  short chain fatty  gas chromatography

摘要:

[目的] 建立水蛭中短链脂肪酸（乙酸、丙酸、丁酸、异丁酸、戊酸、异戊酸、己酸）的含量测定方法。[方法] 采用气相色谱法以2-乙基丁酸和2-乙基己酸为内标物，同时测定水蛭样品中7种短链脂肪酸的含量。采用DB-FFAP弹性石英毛细管柱（30 m×0.25 mm×0.25 μm）程序升温分离各成分，以氢火焰离子化检测器检测（检测器温度：240 ℃），进样口温度：240 ℃，载气：高纯氮气，流速1.0 mL/min；尾吹气：高纯氮气，流速25 mL/min。[结果] 不同批次水蛭样品中各短链脂肪酸含量差异较大，短链脂肪酸总含量范围为1 101~8 571 μg/g，其中乙酸、丙酸、异丁酸、丁酸、异戊酸、己酸的含量范围分别为783.0~3 479、246.7~5 166、0~87.73、0~73.02、0~112.8、0~65.65 μg/g。[结论] 研究建立的水蛭中短链脂肪酸的含量测定方法简便、快速、稳定，可为水蛭的质量控制提供依据。

Abstract:

[Objective] A quantitative analysis method was established for determination of short chain fatty acids (SCFAs) (acetic acid,propionic acid,butyric acid,isobutyric acid,valeric acid,isovaleric acid,and hexanoic acid) in leech by gas chromatography.[Methods] The content of seven SCFAs in leech samples was determined by gas chromatography with 2-ethylbutyric acid and 2-ethylhexanoic acid as internal standards. The components were separated by DB-FFAP elastic quartz capillary column (30 m×0.25 mm×0.25 μm) by programmed rise of temperature, which were detected by hydrogen flame ionization detector. The temperature of inlet and FID detector was fixed at 240℃,respectively. The high purity nitrogen was employed as carrier gas (1.0 mL/min) and make-up gas (25 mL/min) respectively.[Results] The content of SCFAs from different batches leech was obviously different,and the total content of SCFAs was distributed from 1 101 to 8 571 μg/g. Furthermore,the content of acetic acid,propionic acid,isobutyric acid,valeric acid,isovaleric acid,and hexanoic acid was 783.0~3 479,246.7~5 166,0~87.73,0~73.02,0~112.8,0~65.65 μg/g,respectively.[Conclusion] The method established in this study for the determination of SCFAs in leech is simple,rapid and stable,which can provide basis for the quality control of leech.