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| UPLC-Q-Exactive Orbitrap-MS法分析光果甘草地上部分黄酮类成分 |
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张志1, 云鹭1, 侯冰燕1, 刘颖1, 贾栖1, 王文全1,2,3, 侯俊玲1,3, 彭一峰4
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1.北京中医药大学中药学院, 北京 102488;2.中国医学科学院北京协和医学院药用植物研究所, 北京 100193;3.中药材规范化生产教育部工程研究中心, 北京 102488;4.新疆全安药业股份有限公司, 库尔勒 841011
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| 摘要: |
| [目的] 对光果甘草地上部分（茎、叶）的黄酮类化学成分进行分析，为进一步开发利用光果甘草地上部分及阐明其药效物质基础提供科学依据。[方法] 将光果甘草地上部分醇提样品水沉，分为水溶性、脂溶性部位；采用超高效液相色谱-四级杆-静电场轨道阱联用（UPLC-Q-Exactive Orbitrap-MS）法检测样品，使用Xcalibur软件处理分析数据，将得到的成分分子质量、多级图谱碎片信息结合文献、数据库及标准品进行指认。[结果] 从光果甘草地上部分共检测出100个黄酮类成分，有97种在光果甘草地上部分被首次发现；其中水溶性样品检测出61种，脂溶性样品检测出69种。[结论] 通过UPLC-Q-Exactive Orbitrap-MS法，对光果甘草地上部分建立了快速、准确的定性方法，同时证实黄酮类成分含量丰富，为进一步开发利用提供依据。 |
| 关键词: 光果甘草地上部分 黄酮类化学成分 超高效液相色谱-四级杆-静电场轨道阱联用 |
| DOI：10.11656/j.issn.1673-9043.2023.03.12 |
| 分类号:R284 |
| 基金项目:国家工信部中药材提升和保障项目。 |
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| UPLC-Q-Exactive Orbitrap-MS method analysis of the flavonoids in the aerial part of Glycyrrhiza glabra L. |
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ZHANG Zhi1, YUN Lu1, HOU Bingyan1, LIU Ying1, JIA Qi1, WANG Wenquan1,2,3, HOU Junling1,3, PENG Yifeng4
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1.College of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China;2.Institute of Medicinal Plants, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China;3.Engineering Research Center for Standardized Production of Traditional Chinese Materia Medica, Ministry of Education, Beijing 102488, China;4.Xinjiang Quan An Pharmaceutical Co., Korla 841011, China
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| Abstract: |
| [Objective] The flavonoid chemical constituents in the aerial parts(stems and leaves) of Glycyrrhiza glabra L. were analyzed to provide a scientific basis for the further development and utilization of the aerial part of Glycyrrhiza glabra L. and the clarification of its medicinal material basis. [Methods] The alcohol-extracted samples of the aerial parts of Glycyrrhiza glabra L. were precipitated and divided into water-soluble and fat-soluble parts. Samples were tested by ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry(UPLC-Q-Exactive Orbitrap-MS). Data was processed and analyzed using Xcalibur software,and the resulting component molecular mass and multilevel map fragment information were identified in document,database and standards. [Results] A total of 100 flavonoids were detected from the aerial parts of Glycyrrhiza glabra L.,and 97 species were found for the first time in the aerial parts of Glycyrrhiza glabra L.. Among them,61 species were detected in water-soluble samples,and 69 species were detected in fat-soluble samples. [Conclusion] Through UPLC-Q-Exactive Orbitrap-MS liquid chromatography-mass spectrometry technology,a rapid and accurate qualitative method was established for the aerial part of Glycyrrhiza glabra L.,and it was confirmed that the content of flavonoids was abundant,which provided a basis for further development and utilization. |
| Key words: aerial part of Glycyrrhiza glabra L. chemical constituent of flavonoids UPLC-Q-Exactive Orbitrap-MS analysis technology |
