经典名方开心散不同粉碎方式下成分含量差异研究

Study on difference of component content of classic formula Kaixin Powder under different crushing methods

关键词:  开心散  粉碎方式  含量测定  质量控制

Key words:  Kaixin Powder  crushing method  content determination  quality control

摘要:

[目的] 对开心散（KXS）粉碎过程中的温度变化及粉末粒径进行考察，比较由不同粉碎方式处理后KXS中成分的含量差异。[方法] 采用高效液相-二极管阵列检测器法（HPLC-PDA）定量分析5种非挥发性成分，顶空固相微萃取-气质联用技术（HS-SPME/GC-MS）定性定量分析挥发性成分。[结果] 在现代机器粉碎过程中所测温度最高（55 ℃），粉末粒径最小（973.74 nm），传统碾碎较传统捣碎制得的散剂粒径更小，两种方式在温度方面差异不大；HPLC-PDA研究结果表明：5种非挥发性成分中，传统碾碎制得的KXS含量最高，现代机器粉碎制得的KXS含量最低；HS-SPME/GC-MS技术从KXS中共鉴定出56种共有化合物，其中传统捣碎占比93.19%，传统碾碎占比94.16%，现代机器粉碎占比94.04%，可分为8类，其中醚类占比最高（80.38%），萜类种类最多（36种）。[结论] KXS经不同粉碎方式制得后，在成分含量上存在一定差异，其中传统碾碎制备的KXS可较大程度保留其成分。两种检测方法操作简便，实用可靠，为KXS的质量控制研究提供参考。

Abstract:

[Objective] To investigate the temperature change and powder particle size during the crushing process of Kaixin Powder,and to compare the content differences of components in Kaixin Powder after different crushing methods. [Methods] Five non-volatile components were quantitatively analyzed by HPLC-PDA method,and volatile components were qualitatively and quantitatively analyzed by HS-SPME/GC-MS technology. [Results] In the process of modern machine crushing,the temperature measured was the highest(55℃),and the particle size of the powder was the smallest (973.74 nm). The particle size of the powder obtained by traditional grinding is smaller than that of traditional mashing,and the difference in temperature between the two methods is not obvious. HPLC-PDA method shows that:for the five non-volatile components,the content of Kaixin Powder obtained by traditional grinding is the highest,and the content of Kaixin Powder obtained by modern machine crushing is the lowest. A total of 56 common compounds were identified by HS-SPME/GC-MS technology from Kaixin Powder,among which traditional mashing accounted for 93.19%,traditional grinding accounted for 94.16%,and modern machine crushing accounted for 94.04%,can be divided into 8 categories,of which ethers account for the highest proportion(80.38%),and the most terpenoid species(36 species). [Conclusion] There are some differences in the content of ingredients of Kaixin Powder after being prepared by different crushing methods,among which the ingredients of Kaixin Powder prepared by traditional grinding can be largely retained. The two detection methods are simple,practical and reliable,which provide reference for the quality control of Kaixin Powder.