高效液相-蒸发光散射检测器（HPLC-ELSD）测定红参中的精氨酸单糖苷（AF）及精氨酸双糖苷（AFG）含量

Established a new method for the determination of arginyl-fructosyl-glucose and arginyl-fructose in Red gensing by high performance liquid chromatography-evaporative light scattering detector

关键词:  精氨酸双糖苷  精氨酸单糖苷  高效液相-蒸发光散射检测器（HPLC-ELSD）

Key words:  arginyl-fructose  arginyl-fructosyl-glucose  high performance liquid chromatograph y-evaporative light scattering detector（HPLC-ELSD）

摘要:

[目的] 建立一种同时测定人参加工品中精氨酸单糖苷（AF）和精氨酸双糖苷（AFG）含量的方法。[方法] 用Prevail^TM C¹⁸ column （4.6 mm×250 mm，5 μm）色谱柱进行检测。流动相A：色谱乙腈，流动相B：5.0mmol/L七氟丁酸的0.7%三氟醋酸溶液。色谱条件为[0% A（0 min）；0% A（5 min）；15% A（8 min）；35%A（25 min）]；流速0.8 mL/min；漂移管温度为115 ℃；气体流量3.2 L/min。[结果] AF和AFG的检测限分别为0.015和0.010 mg/mL；线性关系相关系数分别为，AF：0.999 7，AFG：0.999 9，表明线性关系良好。AF和AFG检测的精密度，重复性，稳定性以及回收率的相对标准偏差分别为0.43%&0.37%，0.43%&0.55%，0.43%&0.49%，0.45%&0.15%。AF和AFG检测的回收率分别为99.5%&100%，表明方法稳定。经检测，高丽红参，中国红参和生晒参中AF含量分别为0.74%，0.91%和1.14%，AFG含量分别为6.69%，5.12%和0.85%。[结论] 这种方法成功用于高丽红参，中国红参及生晒参中AF及AFG的检测；且红参中AF和AFG含量明显高于生晒参。该方法测定结果可靠，缩短了测定时间，较少杂质干扰。但因为本研究考察氨基酸种类较少，当衍生物种类较多时，仍需进一步考察其分离度。

Abstract:

A method was developed for simultaneous determination of arginyl-fructose（AF） and arginyl-fructosyl-glucose（AFG） content in ginseng processing products. The determination was performed with Prevail^TM C18 column（4.6 mm×250 mm，5 μm），mobile phase A：chromatographic acetonitrile，mobile phase B：5.0 mmol/L heptafluorobutyric acid in 0.7% trifluoroacetic acid solution，and the chromatographic conditions were 0% A（0 min）；0% A（5 min）；15% A（8 min）；35%A（25 min），the flow rate was 0.8 mL/min，drift tube temperature was 115℃，gas flow was 3.2 L/min. The detection limits of AF and AFG were 0.015 and 0.010 mg/mL，respectively. The correlation coefficients of linear relationship are respectively AF：0.9997 and AFG：0.9999，indicating a good linear relationship. The relative standard deviations of precision，repeatability，stability and recovery of AF and AFG were 0.43%&0.37%，0.43%&0.55%，0.43%&0.49%，0.45%&0.15%，respectively. The recoveries of AF and AFG were 99.5% and 100% respectively，indicating that the method was stable. The content of AF in red ginseng，Chinese red ginseng and sun-cured ginseng was 0.74%，0.91% and 1.14%，and the content of AFG was 6.04%，5.08% and 0.85%，respectively. The method was successfully applied to the determination of AF and AFG in red ginseng，Chinese red ginseng and sun-cured ginseng. The content of AF and AFG in red ginseng was significantly higher than that in sun-cured ginseng. The advantages of this method were reliable measurement results，shorter measurement time and less interference from impurities. However，due to the small number of amino acids studied in this study，such as the variety of derivatives，it is still necessary to further investigate the separation degree.