乌梅木瓜汤对菌群失调小鼠肠道菌群及消化酶活性的影响

Effects of Wumei Papaya Decoction on the gutmicrobiota and enzyme activity in mice with dysbiosis

关键词:  乌梅木瓜汤  肠道菌群  高通量测序  消化酶

Key words:  Wumei Papaya Decoction  gut microbiota  high-throughput sequencing  digestive enzyme

摘要:

[目的] 研究乌梅木瓜汤对抗生素诱导的肠道菌群失调小鼠肠道菌群及消化酶活性的影响。[方法] 将32只BALB/c小鼠随机分为4组，分别为正常组、模型组及乌梅木瓜汤低、高剂量给药组，每组8只。除正常组外其余三组小鼠均采用混合抗生素灌胃8 d诱导肠道菌群失调模型。造模成功后，乌梅木瓜汤低、高剂量组分别灌胃乌梅木瓜汤[3.77、7.54 g/（kg·d）]连续8 d，正常组与模型组给予等量无菌水，记录小鼠体质量的变化。末次给药后收集各组小鼠粪便，分别采用16S rRNA高通量测序技术、福林酚比色法、DNS比色法检测分析小鼠肠道菌群及酶活性的变化。[结果] 与正常组相比，模型组小鼠肠道菌群多样性显著降低（P<0.01或P<0.05），变形菌门、疣微菌门、克雷伯氏菌属、大肠杆菌-志贺菌属等菌丰度显著升高（P<0.01或P<0.05），厚壁菌门、脱硫菌门、norank_f_Muribaculaceae属、乳杆菌属丰度显著降低（P<0.05），蛋白酶、淀粉酶及纤维素酶活性显著升高（P<0.01）。与模型组相比，乌梅木瓜汤干预明显改善菌群失调小鼠肠道菌群多样性（P<0.05），显著降低蛋白酶、淀粉酶及纤维素酶活性（P<0.01或P<0.05），可显著增加菌群中norank_f_Muribaculaceae属、乳杆菌属等有益菌的丰度，减少变形菌门、克雷伯氏菌属、大肠杆菌-志贺氏菌属等条件致病菌的群落丰度性（P<0.01或P<0.05）。[结论] 乌梅木瓜汤可改善菌群失调小鼠肠道菌群结构及多样性，降低致病菌丰度、升高有益菌丰度，降低肠道消化酶活性，对菌群失调小鼠肠道微生态的平衡具有积极作用。

Abstract:

[Objective] To study the effects of Wumei Papaya Decoction on gut microbiota and enzyme activity of antibiotic-induced dysbiosis mice. [Methods] The gut dysbiosis mice model was induced by gavage of mixed antibiotic solution. The changes of gut microbiota of mice induced by Wumei Papaya Decoction were detected by 16S rRNA sequencing technology，and the enzyme activity in feces was measured by Folin and DNS method. [Results] Compared with the normal group，the gutmicrobiota diversity of the model group was significantly reduced，Proteobacteria，Verrucomicrobiota，Klebsiella，Escherichia-Shigella were significantly increased，Firmicutes，Desulfobacterota，norank_f_Muribaculaceae，Lactobacillus was significantly reduced，and the activity of protease，amylase and cellulase were significantly increased，and the activities of protease，amylase and cellulase were significantly increased. Compared with the model group，the intervention of Wumei Papaya Decoction significantly improved the alpha diversity and beta diversity of mice with dysbiosis，and significantly reduced the enzyme activity. The Wumei Papaya Decoction can significantly increase the abundance of beneficial bacteria such as norank_f_Muribaculaceae and Lactobacillus，and reduce the community abundance of conditional pathogenic bacteria such as Proteobacteria，Klebsiella，Escherichia-Shigella. [Conclusion] Wumei Papaya Decoction can regulate gut microbial diversity and enzyme activity. It is speculated that gut microbiota and digestive enzyme activities may be the potential targets of Wumei Papaya Decoction in improving gastrointestinal function.