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| 经典名方羌活胜湿汤中12种成分的LC-MS/MS定量分析 |
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李鑫1,2,3, 王敏1,2,3, 邢斌1,2,3, 贾欣陶1,2,3, 刘志东1,2,3
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1.天津中医药大学, 组分中药国家重点实验室, 天津 301617;2.天津中医药大学, 现代中药发现与制剂技术教育部工程研究中心, 天津 301617;3.天津中医药大学, 现代中医药海河实验室, 天津 301617
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| 摘要: |
| [目的] 建立液相色谱-质谱联用（LC-MS/MS）分析方法用于测定羌活胜湿汤颗粒中5-O-甲基维斯阿米醇苷、紫花前胡苷、蔓荆子黄素、羌活醇、蛇床子素、二氢欧山芹醇当归酸酯、异甘草苷、甘草苷、绿原酸、异甘草素、甘草素、阿魏酸12种成分的含量。[方法] 色谱柱为ACQUITY UPLC BEH C18柱（2.1 mm×100 mm，1.7 μm），流动相为0.1%的甲酸水溶液和乙腈，梯度洗脱，流速为0.3 mL/min，柱温为30 ℃，进样量为5 μL。采用电喷雾离子源（ESI）、多反应监测模式（MRM）进行正负离子同时扫描。[结果] 羌活胜湿汤颗粒中12种成分在线性范围内线性关系良好，精密度、重复性、稳定性及加样回收率试验均满足要求。[结论] 实验建立的含量测定方法高效、稳定、可靠，可用于羌活胜湿汤颗粒的质量控制研究。 |
| 关键词: 羌活胜湿汤 液相色谱-质谱联用 含量测定 中药多成分分析 |
| DOI：10.11656/j.issn.1673-9043.2024.09.03 |
| 分类号:R284.1 |
| 基金项目:现代中医药海河实验室科技项目(22HHZYSS00 005);国家中医药管理局青年岐黄学者项目。 |
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| Simultaneous determination of 12 components in Qianghuo Shengshi Decoction by liquid chromatography-tandem mass spectrometry |
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LI Xin1,2,3, WANG Min1,2,3, XING Bin1,2,3, JIA Xintao1,2,3, LIU Zhidong1,2,3
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1.State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;2.Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China;3.Haihe Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China
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| Abstract: |
| [Objective] A liquid chromatography-tandem mass spectrometry(LC-MS/MS) method was established for the simultaneous determination of 12 components in Qianghuo Shengshi Decoction granules,including 5-O-methylvisammioside,nodakenin,casticin,notopterol,osthole,columbianadin,isoliquiritin,liquiritin,chlorogenic acid,isoliquiritigenin,liquiritigenin,and ferulic acid. [Methods] The chromatography was performed on an ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7 μm) with a gradient elution of 0.1% formic acid aqueous solution and acetonitrile as mobile phase at the flow rate of 0.3 mL/min. The column temperature was 30℃ and the injection volume was 5 μL. The samples were detected by an electron spray ionization(ESI) in both positive and negative ion modes using the multiple reaction monitoring(MRM) mode. [Results] The linearity of the 12 components in Qianghuo Shengshi Decoction granules was found to be good within their linear ranges. The results of the precision,reproducibility,stability tests,and recoveries met the requirements. [Conclusion] The method established in this experiment is efficient,stable,and reliable,and can be used for the quality control of Qianghuo Shengshi Decoction granules. |
| Key words: Qianghuo Shengshi Decoction liquid chromatography-tandem mass spectrometry content determination multicomponent analysis |
