丹蒌方对冠心病痰瘀互结证大鼠早期“湿化”的影响

Influence of Danlou Prescription on the “dampness” in the early stage of coronary heart disease with blending of phlegm and blood stasis syndrome

关键词:  冠状动脉粥样硬化性心脏病  痰瘀互结证  丹蒌方  早期湿化

Key words:  coronary heart disease  phlegm and blood stasis syndrome  Danlou Prescription  dampness in the early stage

摘要:

[目的] 采用高脂饲料喂养联合冠状动脉左前降支结扎术建立冠状动脉粥样硬化性心脏病（简称冠心病）痰瘀互结证大鼠模型，观察丹蒌方对冠心病痰瘀互结证早期“湿化”的影响。[方法] 采用Wistar雄性大鼠建立冠心病痰瘀互结证大鼠模型，5周末根据术后检测的射血分数（EF）随机将大鼠分为7组：空白组、假手术组、模型组、丹蒌方低剂量组（200 mg/kg）、丹蒌方中剂量组（400 mg/kg）、丹蒌方高剂量组（800 mg/kg）和阿托伐他汀组（10 mg/kg）。分别于5周末、给药4周（10周末）时对各组部分大鼠进行检测，通过观察大鼠血流动力学及心脏形态学、血脂水平、血液流变学、缺血心肌面积[2，3，5-氯化三苯基四氮唑（TTC）染色]和心肌病理损伤[苏木精-伊红（HE）染色]情况评价冠心病痰瘀互结证大鼠模型建立是否成功，并观察丹蒌方给药4周后对冠心病痰瘀互结证大鼠早期“湿化”的改善作用。[结果] 与空白组和假手术组比较，模型组大鼠心功能指标降低（P<0.01），血脂水平升高（P<0.05或P<0.01），血液呈现黏、浓、凝、聚状态，且模型组大鼠心肌组织经TTC染色后可见明显梗死区域；给药4周后，冠心病痰瘀互结证大鼠的心脏功能和结构均得到改善，血脂水平降低，心肌损伤程度减轻，病变范围缩小，间质水肿及炎性细胞浸润减少。[结论] 丹蒌方能够有效改善冠心病痰瘀互结证大鼠早期“湿化”的心脏结构和功能，降低血脂水平，改善血液黏、浓、凝、聚状态，减轻心肌组织间质水肿，抑制炎性细胞浸润。

Abstract:

[Objective] In this study，high fat diet combined with coronary artery left anterior descending artery ligation were used to establish a rat model of coronary heart disease（CHD） with blending of phlegm and blood stasis syndrome to observe the influence of Danlou Prescription（DLP） on the “dampness” in the early stage of CHD with blending of phlegm and blood stasis syndrome. [Methods] In this study，Wistar male rats were established a rat model of CHD with phlegm and blood stasis syndrome. At the end of 5 weeks，rats were randomly divided into 7 groups according to the ejection fraction（EF） after operation，including control group，sham surgery group，model group，DLP group low-dose group（200 mg/kg），DLP medium-dose group（400 mg/kg），DLP high-dose group（800 mg/kg），and atorvastatin group（10 mg/kg）. At the end of 5 weeks，administration for 4 weeks（10 weeks），some samples were taken from rats in each group. Hemodynamics，cardiac morphology，blood lipid levels，and hemorheology，ischemic myocardial area（TTC staining），and myocardial pathological damage（HE staining） were measured to evaluate the establishment of a rat model of CHD with phlegm and blood stasis syndrome，and to observe the improvement of DLP on the “dampness” in the early stage of CHD with blending of phlegm and blood stasis syndrome. [Results] After model group fed with high-fat diet combined with coronary artery left anterior descending artery ligation，compared with control and sham surgery group，which the cardiac function was significantly decreased（P<0.01）. Blood lipid levels were significantly increased（P<0.05 or P<0.01），and the blood was sticky，concentrated，condensed，and poly. And obvious infarct areas were observed after TTC staining of Model group rats myocardial tissue. After 4 weeks of administration，the cardiac function and structure of rats with CHD with phlegm and blood stasis syndrome were effectively improved，and the blood lipid levels decreased significantly，the myocardial injury degree of rats was lighter，the lesion range was smaller，interstitial edema and inflammatory cell infiltration were reduced. [Conclusion] DLP can effectively improve the heart structure and function of the “dampness” in the early stage of CHD with blending of phlegm and blood stasis syndrome. Reduce blood lipid level and improve the state of blood viscosity，concentration，coagulation and aggregation. Alleviate interstitial edema of myocardial tissue，and inhibit inflammatory cell infiltration.