丁公藤超高效液相色谱指纹图谱建立及含量测定

Establishment of UPLC fingerprint and content determination of Erycibe schmidtii Craib

关键词:  丁公藤  指纹图谱  含量测定  质量评价

Key words:  Erycibe schmidtii Craib  fingerprint  content determination  quality evaluation

摘要:

[目的] 建立丁公藤超高效液相色谱（UPLC）指纹图谱及多成分含量测定研究方法，对30批丁公藤药材质量进行评价。[方法] 基于光电二极管阵列超高效液相色谱（UPLC-PDA），采用Agilent Eclipse XDB-C₁₈色谱柱（2.1 mm×100 mm，1.8 μm），以0.1%甲酸水溶液（A）-乙腈（B）为流动相梯度洗脱。建立丁公藤药材UPLC指纹图谱，指认共有峰并对其进行含量测定。[结果] 建立30批丁公藤药材指纹图谱并获得12个共有峰，指认出其中9种化学成分，并在一定浓度范围内与峰面积线性关系良好（r≥0.999），仪器精密度、方法重复性以及供试品溶液稳定性良好，RSD值均小于5.00%，平均加样回收率为95.3%~103.0%。[结论] 实验建立了丁公藤药材的UPLC指纹图谱及多成分含量测定方法，并证明该方法具有稳定性和可靠性，可以用于后续丁公藤药材的质量控制与研究。

Abstract:

[Objective] To establish an ultra-high performance liquid chromatography（UPLC） fingerprint and a multi-component content determination method for Erycibe schmidtii Craib，and to evaluate the quality of 30 batches of this medicinal material. [Methods] Using photodiode array UPLC（UPLC-PDA），separation was performed on an Agilent Eclipse XDB-C₁₈ column（2.1 mm×100 mm，1.8 μm） with gradient elution using 0.1% formic acid in water（A） and acetonitrile（B） as the mobile phase. The UPLC fingerprint of E. schmidtii was established，common peaks were identified，and their contents were determined. [Results] The UPLC fingerprints of 30 batches of E. schmidtii were established，and 12 common peaks were obtained. Nine chemical components were identified，showing good linear relationships with peak areas within certain concentration ranges（r≥0.999）. The instrument precision，method repeatability，and stability of the test solution were satisfactory，with RSD values all below 5.00%. The average sample recovery rates ranged from 95.3% to 103.0%. [Conclusion] The established UPLC fingerprint and multi-component content determination method for Erycibe schmidtii Craib is stable and reliable，and can be used for subsequent quality control and research of this medicinal material.