| 摘要: |
| 目的 探讨莪术二酮通过调控Wnt/β-catenin信号通路对小鼠骨关节炎的影响。方法 30只SPF级雄性C57BL/6小鼠随机分为假手术组（Sham组）、模型组（OA组）和莪术二酮组（ESET组，60 mg/kg），每组10只，采用内侧半月板失稳术构建骨关节炎模型，连续灌胃给药4周。番红O和苏木精-伊红（HE）染色法观察膝关节形态学变化，酶联免疫吸附实验（ELISA）法检测血清中炎症因子[肿瘤坏死因子α（TNF-α）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）]和化学发光法检测氧化应激指标[谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）、丙二醛（MDA）]，免疫组化和蛋白免疫印迹（Western Blot）法检测膝关节Wnt/β-catenin通路关键蛋白的表达水平。结果 与Sham组比较，OA组小鼠膝关节大量炎症细胞浸润，软骨组织损伤严重，血清TNF-α、IL-1β、IL-6、IL-10和MDA水平明显升高（P＜0.01），而SOD和GSH-Px活性降低（P＜0.01），膝关节wnt3a和β-catenin蛋白相对表达水平升高（P＜0.01）。莪术二酮干预后可明显改善上述指标。结论 莪术二酮可能通过调控Wnt/β-catenin信号通路减轻炎症和氧化应激损伤，从而改善骨关节炎。 |
| 关键词: 莪术二酮 骨关节炎 小鼠 Wnt/β-catenin信号通路 |
| DOI：10.11656/j.issn.1673-9043.2025.09.05 |
| 分类号:R285.5 |
| 基金项目:广东省医学科学技术研究基金项目（B2024110，B2024006）。 |
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| The effects of curdione on osteoarthritis in mice based on Wnt/β-catenin signaling pathway |
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HUANG Chenshuo1, CHEN Ju2, LIN Hao3, LIANG Zhen3, ZHENG Jinchang3, GUO Weixiong3, ZENG Li1
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1.School of Chinese Medicine, Macau University of Science and Technology, Macau 999078, China;2.Department of Pharmacy, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China;3.Orthopaedic Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China
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| Abstract: |
| Objective To investigate the effects of curdione on osteoarthritis in mice by regulating Wnt/β-catenin signaling pathway. Methods Thirty SPF grade male C57BL/6 mice were randomly divided into Sham operation group(Sham), model group(OA) and curdione group(ESET, 60 mg/kg), with 10 mice in each group. The osteoarthritis model was constructed by medial meniscus instability. The drug was given by continuous gavage for 4 weeks. The morphological changes of knee joint were observed by saffrine O and HE staining, and serum inflammatory factors(TNF-α, IL-1β, IL-6, IL-10) were detected by ELISA and oxidative stress indexes(GSH-PX, SOD, MDA) were detected by chemiluminescence. The expression levels of key proteins in Wnt/β-catenin pathway were detected by immunohistochemistry and Western Blot. Results Compared with Sham group, a large number of inflammatory cells infiltrated the knee joint of mice in OA group, cartilage tissue was seriously damaged, serum levels of TNF-α, IL-1β, IL-6, IL-10 and MDA were significantly increased(P < 0.01), while the activities of SOD and GSH-PX were significantly decreased(P < 0.01). The relative expression levels of wnt3a and β-catenin in knee joint were significantly increased(P < 0.01). The above indexes can be improved obviously after curdione intervention. Conclusion Curdione may ameliorate osteoarthritis by regulating the Wnt/β-catenin signaling pathway to reduce inflammation and oxidative stress damage. |
| Key words: curdione osteoarthritis mice Wnt/β-catenin signaling pathway |
