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| Establishment and analysis of HPLC-ELSD fingerprint of Radix Astragali |
| Hits 1672 Download times 1322 Received:December 26, 2016 |
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| DOI
10.11656/j.issn.1673-9043.2017.02.16 |
| Key Words
Radix Astragali;fingerprint;cluster analysis;similarity evaluation |
| Author Name | Affiliation | E-mail | | YAN Zhi-xue | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | | | HE Yong-zhi | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | heyongzhi126@126.com | | WANG Ying | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China | |
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| Abstract
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| [Objective] To establish a fingerprint analysis method of Radix Astragali from various batches.[Methods] The fingerprint was performed by HPLC-ELSD. The conditions of chromatography:Grace Apollo C18(250 mm×4.6 mm,5 μm) was used with a mobile phase of CH3CN-H2O,flow rate:1.0 mL/min, tube:60℃, air pressure:30 psi(2.07 slpm), gain:100. [Results] The HPLC fingerprint for Astragali Radix was developed and 17 common peaks were determined from twelve different batches. The average similarity was over 0.96. The 12 samples were classified as 3 clusters by cluster analysis. [Conclusion] A reliable method was provided for controlling the quality of Radix Astragali. |
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