莪术挥发油抑制人肝癌细胞株SMMC-7721生长的实验研究

Experimental research of Ezhu (turmeric rhizome) naphtha on inhibiting in growth of human carcinoma cells SMMC-7721

关键词:  莪术挥发油  肝癌  细胞凋亡  细胞周期

Key words:  VOCAS  hepatocellular carcinoma  cell apoptosis  cell cycle

摘要:

[目的]探讨莪术挥发油对肝癌细胞生长抑制的作用机制。[方法]用荧光显微镜观察药物作用前后细胞形态学的变化,采用噻唑氮蓝(MTT)法检测莪术油对细胞生长的抑制作用,并用流式细胞仪检测细胞周期的阻滞及凋亡率。[结果]MTT结果表明莪术油浓度与细胞生长抑制率成正比。荧光显微镜观察结果显示,1.0mg/mL的莪术挥发油作用24h后可有效诱导肝癌细胞凋亡,细胞形态产生明显的凋亡特征,细胞核凹陷或固缩成均一的致密物。流式细胞仪检测结果表明,莪术油作用后细胞凋亡率可达28.15%,细胞周期被阻滞在S期。[结论]莪术挥发油可抑制肝癌细胞SMMC7721的生长,诱导凋亡及阻滞细胞周期是其抑制生长的重要机制。

Abstract:

[Objective] To discuss the inhibition mechanism of turmeric rhizome naphtha on inhibiting in the growth of human carcinoma cells SMMC-7721.[Methods] Fluorescence microscope was used to observe the cytomorphology changes before and after giving the turmeric rhizome naphtha. The inhibiting effect was detected by (monofetrazolium) MTT (monotetrazoluim) and the cells’ cyclic retardation and apoptosis rate were examined by using flowcytometry.[Results] MTT test showed that the concentration of turmeric rhizome naphtha had a direct correlation with the inhibiting rate of cell-growth. The result of fluorescence microscope showed that 24 h after administration turmeric rhizome naphtha could induce hepatocell apoptosis. The nucleus was shrank and sunken. flowcytometry showed that the cell apoptosis rate was 28.15% and the cell cycle was arrested at S phase.[Conclusion] Turmeric rhizome naphtha can inhibit the growth of hepatocell SMMC-7721.Inducing apoptosis and blocking the cell cycle are the important mechanism of inhibiting effect.