慢性充血性心力衰竭大鼠动物模型制备

A model in rat of chronic congestive heart failure

关键词:  慢性充血性心力衰竭  动物模型  血液动力学

Key words:  chronic congestive heart failure  animal model  hemodynamics

摘要:

[目的]研制慢性充血性心力衰竭大鼠动物模型。[方法]采用腹主动脉缩窄法制作慢性心衰大鼠模型,测定血液动力学指标,以确定慢性心衰模型成功。[结果]模型组心率 (HR)由 (381.000+52.359)次/min上升到(471.000±57.824)次/min(p<0.05);左室舒张时间 (LVET)由 (80.265 +24.021)ms缩短至 (52.546 +22.258)ms,每搏心输出量(SV)和每分心输出量(CO)均显著下降 (p<0.05),提示模型组大鼠手术后3周心脏功能明显下降,而对照组心功能各项指标均无变化。组间比较HR、LVET、CO、SV均有显著性差异(p<0.05)。[结论]模型组造模成功。同时,观察心衰大鼠临床表现明显,电镜下观察大鼠心肌有明显改变。

Abstract:

[Objective] To explore the preparation of the model of chronic congestive heart failure(CCHF)in rat.[Methods] The CCHF models was made by applying abdominal artery constriction.Whether successful or not in model producing was determined by testing the hemodynamic indexes.[Result] The HR in model group ascended from381.00±52.36per minute to471.00±57.84per minute(p<0.05);LEVT shortened from(80.27±24.02)ms to(52.55±22.26)ms.Both SV and CO descended significantly(p<0.05).It showed that the cardiac function descended obviously in model group 3 weeks after operation,while that in the control group had not any changes.The comparisons of HR,LVET,CO and SV between groups showed significant differences(p<0.05).[Conclusion] The model is made successfully.At the same time,obvious clinical syndromes in the CCHF rats are observed.Significant changes in the cardiac muscles of the rats can be observed electromicroscopically.