痛经宁颗粒对痛经大鼠中枢及外周β-EP含量的影响

Effect of Tongjingning Granule on Central and Peripheral β-EP in Rats with PD

关键词:  痛经宁颗粒  原发性痛经  β-EP

Key words:  Tongjingning Granule  PD  β-EP

摘要:

[目的]探讨痛经宁颗粒的镇痛机制及卵巢激素在原发性痛经中的发病机制。[方法]全部大鼠被随机分为正常对照组、模型对照组、西药对照组 (给予阿司匹林)和中药痛经宁组。观察4组大鼠30、60min扭体发生率;并以放免法检测4组动物血清雌二醇(E₂)、孕酮(P)、以及下丘脑、垂体、卵巢、子宫、血浆β-EP含量。[结果]痛经宁组扭体反应发生率明显低于模型组。E₂ 水平与β-EP含量成负相关,P水平与β-EP含量成正相关,(P<0.01)。痛经宁组大鼠下丘脑、垂体、卵巢、子宫、血浆β-EP含量显著高于模型组和西药对照组(P<0.01)。[结论]痛经宁有明显抑制子宫痉挛,缓解子宫肌收缩,解痉镇痛作用 ;卵巢激素参与了原发性痛经的病理发生 ;中药痛经宁颗粒通过调节卵巢激素调节β-EP含量,显示了其中枢性、外周性镇痛作用的可能。

Abstract:

[Objective] To study the mechanisms of Tongjingning(dysmenorrheal-treating)Granule in analgesic function and the changes of ovary hormone in the course of PD(primary dysmenorrheal. [Methods] Rats were divided into three group randomly:control group treated with Aspirin(western medicine),model group with oxytocin and normal control group.The incidence rate of writhe reaction over the period of30min and60min was observed and the serum E₂,P and the content of β-EP in hypothalamus,pituitary,ovary,uterus and plasma were detected by radioimmuassay(RIA). [Results] The incidence rate of writhe reaction in Tongjingning treated group is lower significantly than that of model group.Nega-tive correlation was found between E₂ and β-EP.Positive correlation,however,was obtained between p and β-EP(P<0.01).The contents of β-EP in hypothalamus,pituitary,ovary,uterus and plasma in Tongjingning group were higher than that of model group and Aspirin group(P<0.01). [Conclusion] Tongjingning has a function of restraining convulsion and alleviating contraction of uterus muscle,can relieve convulsion and has analgesic effect.Ovary hormone is related to the pathologic occurring of PD.Tonjingning has a central and peripheral analgesic functions and can adjust the content of β-EP possibly by regulating ovary hormone.