鱼腥草对糖尿病大鼠肾脏组织中BMP-7和TGF-β1表达的影响

Influence of Houttuynia Cordata Thunb on Expression of BMP-7 and TGF-β1 in Kidney Tissue of Diabetic Rats

关键词:  鱼腥草  糖尿病  肾脏  转化生长因子β1  骨形成蛋白-7

Key words:  houttuynia cordata thunb  diabetes  kidney  transform growth factor-β1  bone morphogenetic protein-7

摘要:

[目的]探讨鱼腥草对糖尿病肾脏组织中转化生长因子β1(TGF-β1)和骨形成蛋白-7(BMP-7)表达的影响。[方法]建立链脲佐菌素(STZ)诱导的糖尿病模型,随机分为模型组、鱼腥草组和洛汀新组,另设正常组;观察8周后肾质量/体质量比值、肾小球面积及24h尿β₂微球蛋白、24h尿白蛋白和肌酐清除率,并应用免疫组化检测肾脏组织中TGF-β1、BMP-7的蛋白表达。[结果]鱼腥草给药可明显抑制糖尿病模型大鼠肾小球的肥大,降低24h尿β₂微球蛋白、24h尿白蛋白排泄率和肌酐清除率(P<0.05);免疫组化半定量显示,与模型组比较,鱼腥草组TGF-β1表达明显减少(P<0.05),BMP-7表达增多(P<0.05),而与洛汀新组比较无显著差异(P>0.05)。[结论]鱼腥草对糖尿病肾脏组织有明显的保护作用,其机制可能与其降低肾脏中TGF-β1表达,增加BMP-7的表达有关。

Abstract:

[Objective] To explore the effect of Houttuynia cordata Thunb(HCT)on the expression of transform growth factor β1(TGF-β1)and bone morphogenetic protein-7(BMP-7)of renal tissue in diabetic rats.[Methods] Diabetes in rats was induced by intravenous injection of streptozotocin(STZ).They were randomly divided into model group,HCT group and lotensin group and normal control group.Eight weeks later the ratio of kidney weight to body weight,renal glomerular area,the excretion of β₂-microglobin in urine collected for 24 hours,the clearance ratio(CCr)of albumin and creatinine in urine collected for 24 hrs were determined.The expression of TGF-β1 and BMP-7 in renal tissue was determined with immunohistochemistry.[Results] Administration of HCT could markedly inhibit the enlargement of renal glomeruli,decrease the excretory rate of β₂-microglobin and urinary albumin and creatinine in urine collected for 24 hrs(CCr).The renal expression of TGF-β1 in HCT group decreased remarkably(P<0.05)while and BMP-7 increased obviously(P<0.05)in comparison with those in model group.There was no significant difference between HCT and lotensin groups(P>0.05).[Conclusions] Houttuynia cordata Thunb may protect against the renal lesion in diabetic rats through inhibiting the expression of TGF-β1 and enhancing the expression of BMP-7.