| 摘要: |
| [目的]观察丹酚酸B(SalB)体外干预骨髓间充质干细胞(MSCs)后细胞形态及心肌特异性蛋白肌钙蛋白T(cTnT)的表达。[方法]培养、纯化及鉴定MSCs,用SalB、5-氮胞苷组(5-aza)、SalB联合5-aza(SalB+5-aza)分别定向诱导分化,观察此过程中MSCs的形态学变化并采用免疫细胞化学法鉴定诱导后MSCs心肌特异性肌钙蛋白T(cTnT)的表达。[结果]诱导后的MSCs体积增大,增殖减慢,并出现肌管样结构;免疫细胞化学结果显示诱导后MSCs表达心肌特异性蛋白cTnT。[结论]SalB在体外定向诱导MSCs分化为心肌细胞的过程中发挥了一定的作用。 |
| 关键词: 丹酚酸B 骨髓间充质干细胞 体外诱导 心肌肌钙蛋白T |
| DOI：10.11656/j.issn.1672-1519.2007.04.30 |
| 分类号: |
| 基金项目:天津市社会发展科技计划项目(编号:043111211) |
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| Expression of cTnT induced by Salvianolic Acid B in differentiation of bone marrow Mesenchymal Stem Cells into myocardium-like cell |
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SUN Lian-sheng1, Xu Xiu-mei2, GUO Mao-juan2
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1.Tianjin Hangzhou Road Hospital in Tanggu District, Tianjin 300000, China;2.Tianjin University of TCM, Tianjin 300193, China
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| Abstract: |
| [Objective] To observe the effect of salvianolic acid B (SalB) in differentiation of bone marrow mesenchymal stem cells (MSCs) into myocardium-like cells. [Methods] MSCs was cultured,purified and evaluated,then MSCs was induced by 5-aza,SalB and 5-aza associating SalB to observe the cells’ morphology change and evaluate the expression of cardiac troponin T (cTnT) by immunocytochemistry method. [Results] After induction,the volume of MSCs increased,the proliferation speed decreased. Myotube-like structure appeared and expressed cTnT. [Conclusions] SalB plays a certain role in the process of induction into myocardium-like cells. |
| Key words: SalB MSCs induction in vitro cTnT |
