| 摘要: |
| [目的]进一步从调节γ-氨基丁酸(GABA)代谢的角度探讨小儿抗痫胶囊的作用机制。[方法]采用高效液相色谱-紫外检测法,观察大鼠大脑皮质、双侧海马的γ-氨基丁酸含量和谷氨酸(GLU)含量的变化。[结果]戊四唑点燃癫痫大鼠脑内GABA的含量降低,GLU含量升高;经小儿抗痫胶囊治疗后,中药高剂量组皮质、海马GABA含量较模型组均有显著升高,中药高剂量组皮质、海马GLU含量均显著低于模型组,表明小儿抗痫胶囊有良好增加模型大鼠脑内GABA含量,降低GLU含量而抗惊厥的作用;而中药高剂量组与低剂量组比较有显著性差异,表明小儿抗痫胶囊对GABA、GLU含量的影响还与剂量显正相关。[结论]小儿抗痫胶囊能显著抑制大鼠戊四唑点燃发作,其疗效与药物剂量呈正相关,作用机制可能与其降低大脑GLU含量,增加大脑GABA含量,影响GABA代谢有关。 |
| 关键词: 小儿抗痫胶囊 癫痫 戊四唑点燃模型 γ-氨基丁酸 谷氨酸 |
| DOI：10.11656/j.issn.1672-1519.2007.04.31 |
| 分类号: |
| 基金项目:天津市卫生局中医、中西医结合科研基金资助项目(200039) |
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| Experiment study of Pedo-Anti-epilepsy Capsule on γ-GABA and GLU content in the brain of epilepsy rats |
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TIAN Shu-xia1, LI Xin-min2
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1.Tianjin University of TCM, Tianjin 300193, China;2.The First Affiliateed Hospital to Tianjin University of TCM, Tianjin 300193, China
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| Abstract: |
| [Objective] To observe the change of γ-GABA and Glu content in the brain of epilepsy rats based on the research and explore the effect mechanism of Pedo-anti-epilepsy capsule. [Methods] To observe change of γ-GABA and Glu content in the cerebral cortex and hippocamp of rats by using HPLC and ultra-violet detection method. [Results] The Pedo-anti-epilepsy Capsule had obviously antagonism for pentylenetetrazol lighting and attacking of rats. [Conclusions] The mechanism of action may have related with the change of γ-GABA and GLU content and GABA metabolism in the brain. |
| Key words: Pedo-anti-epilepsy Capsule epilepsy pentylenetetrazol lighting model γ-GABA GLU |
