| 摘要: |
| [目的]筛选香加皮的抗肿瘤活性部位。[方法]用大孔吸附树脂柱层析法对香加皮的和60%乙醇回流提取物进行分段处理,利用四甲基偶氮唑盐(MTT)法测定各提取部位体外抗肿瘤活性。[结果]70%乙醇洗脱部位对体外肿瘤细胞表现出抑制活性,并在测定浓度范围内呈现出良好剂量依赖性。95%乙醇洗脱部位的抑制作用较弱。而水洗脱物和20%乙醇洗脱物则未呈现出抗肿瘤活性。在各提取部位中70%乙醇提取物活性最强。[结论]70%乙醇提取部位是香加皮主要的体外抗肿瘤活性部位。 |
| 关键词: 香加皮 抗肿瘤活性 四甲基偶氮唑盐 |
| DOI：10.11656/j.issn.1672-1519.2008.02.30 |
| 分类号: |
| 基金项目:天津市高等学校科技发展基金计划项目(20070302);天津市科技创新专项资金项目(06FZZDSH004011)资助 |
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| Antitumor activity of extract from different parts of Periploca Sepium Bge. |
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LIU Yang1, LIU Hong2, WANG Xiao-ying2
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1.Research College of TCM, Engineering Research Center of Modern Chinese Medicine Discovery and Preparation Technique, Tianjin University of TCM, Tianjin 300193, China;2.Tianjin University of TCM, Tianjin 300193, China
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| Abstract: |
| [Objective] To screen the part of Periploca Sepium Bge.(PSBF) with antitumor activity.[Methods] The extract of Periploca Sepium Bge.with 60% ethanol was treated in the way of macroporous adsorptive resins column chromatography to afford different fractions.The antitumor activities of the different fractions in vitro were determined by MTT assay.[Results] The 70% ethanol elution(PSBF-C) showed strong inhibitory effect on growth of cancer cells with significant dosage-dependence.The 95% ethanol elution(PSBF-D) showed low antitumor activity in vitro.And the most sensitive tumor cell lines to PSBF-C and PSBF-D were human hepatocellular carcinoma SMMC-7721 cell line.Whereas the fraction of 20% ethanol/H2O elution showed no antitumor activity.[Conclusions] The extract of 70% ehanol elution was the principal antitumor fraction from Periploca Sepium Bge. |
| Key words: Periploca Sepium Bge Antitumor activity MTT |
