心脑舒通胶囊对急性脑缺血再灌注大鼠皮质单胺类神经递质的影响

Effect of Xinnaoshutong capsule on monoamine neurotransmitter in Cortex in rats with cerebral ischemia and reperfusion

关键词:  急性脑缺血再灌注  病理形态改变  单胺类神经递质  心脑舒通胶囊

Key words:  acute cerebral ischemia and reperfusion  pathomorphology  monoamine neurotransmitter  Xinnaoshutong capsule

摘要:

[目的]观察活血化瘀通络中药——心脑舒通胶囊对急性脑缺血再灌注大鼠皮质单胺类神经递质的影响。[方法]健康雄性SD大鼠42只,动物随机分为假手术组、模型组和心脑舒通组,心脑舒通组灌胃给予心脑舒通混悬液,假手术组和模型组给予等量蒸馏水。术前3d开始连续灌胃给药(11.57mg/g),1次/d,至术后1d共4d。采用线栓法致大鼠大脑中动脉阻塞建立急性脑缺血再灌注模型,缺血1.5h再灌注24h后断头取脑,常规苏木-伊红(HE)染色观察患侧皮质病理学改变,高效液相色谱法测定患侧皮质单胺类神经递质含量变化。[结果]心脑舒通胶囊能明显减轻急性脑缺血再灌注大鼠皮质病理形态改变,显著降低异常升高的肾上腺素(E)、多巴胺(DA)、5-羟色胺(5-HT)的含量。[结论]心脑舒通胶囊可减轻脑缺血损伤程度,其作用机制可能与改善单胺类神经递质的紊乱有关。

Abstract:

[Objective] To research the effect of Xinnaoshutong(dredging heart and brain) capsule (XNST), a Chinese medicine with effect of activating blood circulation,removing stasis and dredging collateral), on monoamine neurotransmitter in rats with cerebral ischemia and reperfusion injury. [Methods] Forty-two male SD rats were randomly divided into sham,model,XNST treatment group. Acute cere-bral ischemia and reperfusion was induced with thread occlusion technique inducing middle cerebral artery occlusion in rats. Changes of cortical neurons was determined by HE staining and the concentrations of the monoamine neurotransmitters was measured by HPLC. [Results] Xinnaoshutong capsule had following effects:alleviate the pathological injury and reduce contents of E,DA and 5-HT. [Conclusion] Xinnaoshutong capsule can alleviate the change of pathological injury and the mechanism, underlying the possible protective mechanism is to improve disturbed neurotransmitters.