| 摘要: |
| [目的]建立杠柳细胞悬浮培养体系,并测定悬浮细胞中杠柳毒苷和4-甲氧基水杨醛含量。[方法]无菌条件下切取杠柳幼根诱导愈伤组织,将其进行继代增殖培养,然后再将愈伤组织接种到液体培养基中建立细胞悬浮培养体系,并测定细胞中杠柳毒苷和4-甲氧基水杨醛含量。[结果]建立了杠柳细胞悬浮培养体系,并测定了细胞中杠柳毒苷和4-甲氧基水杨醛含量分别为4.06μg/g和1.92μg/g。[结论]通过组织培养方式可以成功建立杠柳细胞悬浮培养体系,细胞生长旺盛,但细胞中有效成分含量低,需要进一步对其基本培养基和培养条件进行考察。 |
| 关键词: 杠柳 细胞悬浮培养体系 杠柳毒苷 4-甲氧基水杨醛 |
| DOI：10.11656/j.issn.1672-1519.2010.02.37 |
| 分类号: |
| 基金项目:天津市科技支撑计划重点项目(09ZCKFSH01100) |
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| Establishment of the cell suspension culture system of periploca sepium bunge and content measurement of the effective constituent of periplocin and 4-methoxysalicylaldehyde |
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ZHANG Jian,GAO Wen-yuan,WANG Juan
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1.Tianjin University of TCM, Tianjin 300193, China;2.Tianjin University of Science and Technology, Tianjin 300457, China
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| Abstract: |
| [Objective] To establish the Cell Suspension Culture System of Periploca sepium Bunge and measure the constituent of Periplocin and 4-Methoxy salicylaldehyde. [Methods] Cut and collected the root and induce callus under sterile conditions. Then,propagate the callus for several cycles. Select eugonic and incompact callus and inoculate them to liquid medium to establish the System of Cell Suspension Culture. Then,measure the content of Periplocin and 4-Methoxysalicylaldehyde in cell. [Results] The Cell Suspension Culture System of Periploca sepium bunge was established and the constituent of periplocin and 4-Methoxysalicylaldehyde was measured as 4.06 μg/g and 1.92 μg/g-respectively. [Conclusion] The Cell Suspension Culture System of Periploca sepium Bunge can be established successfully by tissue culture,but the effective constituent of the cell was low,so it was necessary to study the minimum medium and culture conditions further. |
| Key words: periploca sepium bunge system of cell suspension culture minimal medium periplocin 4-methoxysalicylaldehyde |
