| 摘要: |
| [目的]建立一个紫外可见分光光度法,对不同产地柴胡样品中的多糖进行含量测定。[方法]柴胡多糖含量测定采用苯酚-硫酸显色,487nm为检测波长,对提取过程的主要步骤及方法学进行实验,建立其测定方法。[结果]柴胡多糖的的测定方法简便、准确、稳定,线性范围为10.484～75.936μg,(r=0.9986),11个不同产地的柴胡中多糖含量范围在46.299～96.965g/kg生药。[结论]本研究建立的柴胡多糖含量测定方法简便易行、稳定、可靠。 |
| 关键词: 柴胡多糖 含量测定 紫外可见分光光度法 |
| DOI：10.11656/j.issn.1672-1519.2010.04.36 |
| 分类号: |
| 基金项目: |
|
| Quantitative analysis of polysaccharide in radix bupleuris from different areas by ultraviolet visible spectrophotometry |
|
LI Di-hua, LIU Jun-hong, WU Xiao-xian
|
|
Tainjin Nankai Hospital, Institute of Acute Abdomen by Integrated Medicine, Tianjin 300100, China
|
| Abstract: |
| [Objective] To set up an ultraviolet visible spectrophotometry(UV) method to determine the content of polysaccharide in herbs obtaining from different areas. [Methods] The content of Polysaccharide was tested with the color appearing in the phenol-sulphuricacid reagent.The testing wavelength was 478nm.Its UV assaying method was found by experimenting on the key steps and methodology of extraction. [Results] UV assay method for Polysaccharide was simple and convenient,accurate,and stable.Its linearity range was 10.484～75.936μg.Among the Radix bupleuris from 11 places of origin,the content range of Polysaccharide per 1 gram of crude drug was 46.99～96.965mg. [Conclusion] The method was simple and easy to perform and the obtained results were stable and reliable. |
| Key words: Polysaccharide quantitative analysis ultraviolet visible spectrophotometry |
