| 摘要: |
| [目的]研究熄风胶囊对氯化锂-匹罗卡品癫痫大鼠皮质和海马多药耐药相关蛋白1(MRP1)表达的影响。[方法]1)利用氯化锂-匹罗卡品制作癫痫大鼠模型。2)实验大鼠按随机数字表法随机分为正常组、模型组、熄风胶囊高剂量组(熄高组)、熄风胶囊中剂量组(熄中组)、熄风胶囊低剂量组(熄低组)、熄风胶囊大剂量+卡马西平(CBZ)组(联高组)、熄风胶囊大剂量+CBZ1/2剂量(联低组)和CBZ组。3)通过Western-blot方法检测癫痫大鼠海马和皮质MRP1的表达程度。[结果]实验结果显示治疗组与模型组之间比较差异有统计学意义(P<0.05)。[结论]熄风胶囊能有效抑制MRP1的过度表达。 |
| 关键词: 难治性癫痫 熄风胶囊 匹罗卡品 多药耐药相关蛋白1 |
| DOI：10.11656/j.issn.1672-1519.2012.02.19 |
| 分类号: |
| 基金项目:天津市应用基础及前沿技术研究计划项目(08JCYBJC10900) |
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| Effect of Xi Feng capsule on expression of MRP1 of hippocampus and cortex in epileptic rats induced by lithium-pilocarpine |
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LI Xin-min, REN Yan-yan, CHEN Hui, LU Yan-li
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The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China
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| Abstract: |
| [Objective]To study the intervention effect of Xi Feng capsule on medicine-related protein 1(MRP1)of hippocampus and cortex in epileptic rats induced by Lithium-Pilocarpine.[Methods]1)Epilepsy was duplicated in rats with lithium-pilocarpine chemical ignition.2)All the experimental rats were randomly divided into eight groups:normal control group,model group,Xi Feng interventiongroup(Xi Feng high-dose group),Xi Feng intervention group(Xi Feng middle-dose group),Xi Feng intervention group(Xi Feng low-dose group(Xi Feng high-dose group),Xi Feng intervention group(Xi Feng middle-dose group),Xi Feng intervention group(Xi Feng low-dose group),Xi Feng high-dose+CBZ group,Xi Feng high-dose+CBZ 1/2 dose group,CBZ group.3)The expression of MRP1 of hippocampu and cortex in epileptic rats was observed by Western-blot.[Results]The result of Western-blot method suggested that there was a significant difference between intervention group of Xi Feng capsule and model group(P<0.05).[Conclusion]Xi Feng capsule single and combined with CBZ could efficiently depress the overexpression of MRP1 of the hippocampus and cortex in epileptic rats induced by Lithium-Pilocarpine. |
| Key words: intractable epilepsy Xi Feng capsule pilocarpine MRP1 |
