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| 犀黄丸对大肠癌LoVo细胞基质金属蛋白酶2,9蛋白表达的影响 |
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孙莉娜1, 孟静岩1, 王威1, 应森林1, 李丹1, 马佐英1, 贾彦焘2
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1.天津中医药大学,天津 300193;2.天津中医药大学第一附属医院,天津 300193
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| 摘要: |
| [目的] 研究犀黄丸含药血清对大肠癌LoVo细胞中基质金属蛋白酶-2,9(MMP-2,MMP-9)表达的影响及意义.[方法] 应用酶联免疫吸附试验(ELISA)方法,检测犀黄丸含药血清作用下LoVo细胞培养上清液中MMP-2、MMP-9的蛋白表达水平.[结果] 在犀黄丸含药血清的干预下,LoVo细胞中MMP-2、MMP-9的蛋白分泌水平与空白对照组比较明显下调,差异具有统计学意义(P < 0.05).[结论] 犀黄丸可能通过抑制MMP-2和MMP-9的蛋白表达,达到抑制大肠癌转移的作用. |
| 关键词: LoVo细胞 犀黄丸 含药血清 基质金属蛋白酶-2,9 |
| DOI：10.11656/j.issn.1672-1519.2012.04.23 |
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| 基金项目:国家自然科学基金项目(3087227). |
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| Effect of Xihuang pills on protein expressions of MMP-2 and MMP-9 in human colorectal carcinoma LoVo cell |
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SUN Li-na1, MENG Jing-yan1, WANG Wei1, YING Sen-lin1, LI Dan1, MA Zuo-ying1, JIA Yan-tao2
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1.Tianjin University of TCM, Tianjin 300193, China;2.The First Affiliated Hospital of Tianjin University of TCM, Tianjin 300193, China
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| Abstract: |
| [Objective] To investigate the effect of Xihuang pills(medicine-contained serum) on the protein expressions of matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9(MMP-9) in human colorectal carcinoma LoVo cell. [Methods] The enzyme-linked immunoassay (ELISA) was used to determine the expression of MMP-2 and MMP-9 in the culture solution of LoVo cell under the action of Xihuang pills. [Results] The expression of MMP-2 and MMP-9 in medication groups was significantly lower than those of control group (P < 0.05). [Conclusion] Xihuang pill has a potent effect of reducing the expressions of MMP-2 and MMP-9. This mechanism may play an important role in the suppression of colorectal carcinoma metastasis. |
| Key words: LoVo cell Xihuang pills medicine-contained serum matrix metalloproteinase-2, 9 |
