三个不同品种甘草多糖的含量测定

Content determination of polysaccharides in Radix Glycyrrhizae from three different species

关键词:  甘草多糖  苯酚-硫酸法  定量测定

Key words:  Radix Glycyrrhizae polysaccharides  Phenol-sulfuric acid colorimetry method  quantitative determination

摘要:

[目的] 建立甘草多糖含量定量的测定方法,为评估甘草药材的质量提供方法。[方法] 甘草样品经95%乙醇除去小极性的杂质后,采用水提醇沉的方法,再通过多步除杂质得精制多糖。采用苯酚-硫酸法测定甘草多糖与葡萄糖的换算因子,并以此测定不同品种甘草药材中多糖的含量。[结果] 三个不同品种间甘草生药中甘草多糖的含量为光果>胀果>乌拉尔。乌拉尔甘草多糖平均加样回收率为98.23%,RSD为1.63%.[结论] 此方法简便可行,甘草多糖供试液在4 h内显色稳定,重复性较好,平均加样回收率高,可作为甘草多糖的含量测定方法。

Abstract:

[Objective] An phenol-H₂SO₄ colorimetry method was developed for the determination of polysaccharides in Radix Glycyrrhizae samples from different species in order to provide a scientific basis for a quality evaluation of Materia Medica. [Methods] The samples were extracted with 95% ethanol to remove the little polarity interference components during the pretreatment process, then water extraction and alcohol precipitation were performed, then the obtained polysaccharides were refined by passing many processes. A corrected factor was used to minimize the error between determinations of glucose and polysaccharides. The contents of the polysaccharides in Radix Glycyrrhizae of different samples were determined by phenol-H₂SO₄ colorimetry method. [Results] The content of three different species' raw material polysaccharides was Radix Glycyrrhizae Glabrae >Radix Glycyrrhizae Inflatae >Glycyrrhiza uralensis. The average recovery rate for the Glycyrrhiza uralensis polysaccharides was 98.23% with 1.63% of RSD (n=6). [Conclusion] This determination method is simple, practical, with good reproducibility, high average recovery rate and the color of the treated samples are stabilized within 4h. So this method can be used to determine of Radix Glycyrrhizae polysaccharides.