HPLC法同时测定金银花及金芪降糖片中9种成分的含量

Simultaneous determination of nine components in Lonicerae Japonicae Flos and Jinqi Jiangtang tablet by HPLC

关键词:  金芪降糖片  金银花  HPLC  新绿原酸  隐绿原酸  异绿原酸A

Key words:  Jinqi Jiangtang tablet  Lonicerae Japonicae Flos  HPLC  neochlorogenic acid  cryptochlorogenic acid  isochlorogenic acid A

摘要:

[目的] 建立高效液相色谱（HPLC）法同时测定金银花及金芪降糖片中新绿原酸、隐绿原酸、绿原酸、咖啡酸、木犀草苷、芦丁、金丝桃苷、异绿原酸A、异绿原酸C 9种成分的含量。[方法] 色谱柱为Waters SymmetryShieldTM RP18柱（250 mm×4.6 mm，5 μm），流动相为乙腈（A）-水（0.1%磷酸，B），线性梯度洗脱：0～5 min，10%～13% A；5～20 min，13%～15% A；20～30 min，15%～18% A；30～45 min，18%～20% A；45～52 min，20%～23% A；52～70 min，23% A，体积流量1.0 mL/min，检测波长327 nm；柱温25℃。[结果] 9种成分均能达到基线分离，线性关系良好，加样回收率在95%～105%.在该方法下测定了16批金银花药材、11批金芪降糖片中9种成分的含量。[结论] 该法专属性强、灵敏度高、重复性好、简便易行，可用于同时测定金银花及金芪降糖片中新绿原酸、隐绿原酸、绿原酸、咖啡酸、木犀草苷、芦丁、金丝桃苷、异绿原酸A、异绿原酸C 9种成分的含量，可为金芪降糖片的现代制剂研究和质量控制提供依据。

Abstract:

[Objective] To develop a HPLC method for simultaneous determination of neochlorogenic acid, cryptochlorogenic acid, chlorogenic acid, caffeic acid, cynaroside, rutin, hyperoside, isochlorogenic acid A and isochlorogenic acid C in Lonicerae Japonicae Flos and Jinqi Jiangtang tablet. [Methods] The chromatographic conditions were follows: Waters Symmetry Shield TM RP18 column (250 mm×4.6 mm, 5 μm), acetonitrile (A) and water ( 0.1% phosphoric acid, B) as mobile phases for gradient elution, 0～5 min,10%～13% A, 5～20 min, 13%～15% A;20～30 min,15%～18% A;30～45 min,18%～20% A;45～52 min,20%～23% A;52～70 min,23% A; the flow rate being 1.0 mL/min. The detection wavelength was set at 327 nm and the column temperature was 25℃。 [Results] The results showed that nine components were well separated and showed good linearity. The average recoveries were between 95%～105%. [Conclusion] This is a specific, sensitive, repeatable and simple method for simultaneous determining neochlorogenic acid, cryptochlorogenic acid, chlorogenic acid, caffeic acid, cynaroside, rutin, hyperoside, isochlorogenic acid A and isochlorogenic acid C in Lonicerae Japonicae Flos and Jinqi Jiangtang tablet. The method can be available for the quality control of Jinqi Jiangtang tablet.