大孔树脂纯化葛根的使用次数和再生方法

Life and intensified regeneration technology of macroporous resins in extracting and purifying puerarin and total flavonoids from pueraria lobata

关键词:  大孔树脂  葛根  总黄酮  再生

Key words:  macroporous resin  puerarin  total flavonoids  regeneration

摘要:

[目的] 研究HPD-200A型大孔树脂在纯化葛根素及葛根总黄酮时的使用寿命和再生方法。[方法] 以葛根总黄酮和葛根素的吸附容量和精致后的纯度为指标，考察大孔树脂在多次吸附洗脱和再生后的吸附洗脱能力的变化。[结果] 树脂初次使用时，葛根素吸附容量约为17.29 g/L，总黄酮的吸附容量约为46.00 g/L，两者的纯度为32.40%和 75.52%，连续使用11次后葛根素吸附容量为原来的82.26%，总黄酮的吸附容量为原来的80.99%，两者纯度为27.23%和67.99%，纯度略有降低。用95%乙醇再生处理后，HPD-200A大孔树脂对葛根素和总黄酮的吸附容量分别可达到新树脂的94.35%和91.87%，两者的纯度也均可达到新树脂的97%以上。[结论] 使用HPD-200A型大孔树脂精制葛根时，新树脂可连续使用11次，用95%乙醇再生后，至少可连续使用3次，即一批HPD-200A型树脂至少可使用14次，本研究为葛根纯化工艺的产业化确定提供了实验基础和参考。

Abstract:

[Objective] To study the life and intensified regeneration technology of HPD-200A macroporous resins in purifying puerarin and puerarin flavonoids. [Methods] With the adsorption capacity and purity of puerarin and total flavonoids as indexes, the absorption capacity of HPD-200A macroporous resins which were multiple absorbed and intensified regenerated was studied. [Results] First, the adsorption capacity of puerarin and total flavonoids was about 17.29 g/L and 46.00 g/L, respectively. Their adsorption capacity became 82.26% and 80.99% of the original and the purity was 27.23% and 67.99% after HPD-200A macroporous resin was used continuously for 11 times. With 95% glycol regeneration, the adsorption capacity of HPD-200A macroporous resins for puerarin and total flavonoids could reach above 94.35% and 91.87%, the purity was more than 97% of the new resin. [Conclusion] With HPD-200A macroporous resin refining puerarin, new resin can be used for 11 times continuously. When regenerated with 95% ethanol, it can be used at least three times again, that is, the HPD-200A resin can be used at least for 14 times.