| 摘要: |
| [目的] 研究康肾口服液用高效液相色谱法（HPLC）测定其人参皂苷的含量。[方法] 以十八烷基硅烷键合硅胶为填充剂，以乙腈-水（18：82）为流动相，检测波长为203 nm.理论塔板数按人参皂苷Rg1峰计算应不低于6 000.[结果] 人参皂苷Rg1浓度在0.053～0.265 g/L范围内，人参皂苷Re浓度在0.037～0.185 g/L范围内，峰面积与浓度线性关系良好，平均回收率为99.12%.[结论] HPLC法测定康肾口服液中人参皂苷的含量，操作简单、重复性好，可有效控制康肾口服液的质量标准。 |
| 关键词: HPLC 康肾口服液 人参皂苷 含量测定 |
| DOI：10.11656/j.issn.1672-1519.2014.03.17 |
| 分类号: |
| 基金项目:河南省科学技术厅2009年科技发展计划（092102310202）. |
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| HPLC method for determining ginsenoside content in Kangshen oral liquid |
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HAO Shao-jun, LI Wen-jun, FAN Li-li, ZHANG Zheng-chen
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The 371st Hospital Center of Chinese People's Liberation Army, Xinxiang 453000, China
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| Abstract: |
| [Objective] To study the content of the ginsenoside in Kangshen oral liquid by HPLC method. [Methods] Take eighteen alkyl silane bonded silica as filler, water solution with acetonitrile (82:18) as mobile phase; the detection wavelength was 203 nm. Number of theoretical plates by ginsenoside Rg1 peak calculation should not be less than 6000. [Results] The ginsenoside Rg1 concentration in the range of 0.053~0.265 g/L, ginseng saponin Re concentration in the range of 0.037~0.185 g/L, the peak area and the concentration had good linear relationship. The average recovery rate was 99.12%. [Conclusion] The HPLC method for the determination of ginsenoside content in Kangshen oral liquid has a feature of simple operation, good reproducibility. It can effectively control the quality standard of Kangshen oral liquid. |
| Key words: HPLC Kangshen oral liquid ginsenoside content determination |
