参麦注射液对异丙肾上腺素诱导大鼠心肌缺血的保护作用

Protective effect of Shenmai injection against myocardial ischemia injury induced by isoproterenol in rats

关键词:  参麦注射液|异丙肾上腺素|心肌缺血

Key words:  Shenmai injection|isoproterenol|myocardial ischemia injury

摘要:

[目的] 研究参麦注射液对异丙肾上腺素诱导大鼠心肌缺血的保护作用。[方法] 皮下多点注射异丙肾上腺素（ISO）85mg/kg，每日1次，连续2d，制备大鼠心肌缺血模型。记录大鼠心电图（ECG）的变化，观察血流动力学、超声心动图及心肌组织病理学的改变，并测定血清肌酸激酶（CK）、乳酸脱氢酶（LDH）、超氧化物歧化酶（SOD）及丙二醛（MDA）的活性以及心脏指数的改变。[结果] 参麦注射液能够降低由ISO所致的心肌缺血大鼠ST段的抬高，明显改善左室功能，降低心肌组织病变程度，并且显著降低CK、LDH、MDA水平及心脏指数，增加SOD水平，与模型组比较有统计学意义（p<0.05或p<0.01）。[结论] 参麦注射液对ISO诱导的大鼠心肌缺血有一定的保护作用。

Abstract:

[Objective] To study the protective effect of Shenmai injection (SMI) against myocardial ischemia injury induced by isoproterenol in rats. [Methods] A rat model of myocardial ischemia was established by subcutaneous injections of isoproterenol (85 mg/kg for two consecutive days). The rat electrocardiogram (ECG) was recorded, the changes of hemodynamics, echocardiography and myocardial histopathology were also observed. Activities of creatine phosphokinase (CK), lactate dehydrogenase (LDH), the superoxide diSMIutase (SOD) and the levels of malondialdehyde (MDA) in rat serum were determined, heart index were measured simultaneously. [Results] In comparison with model group, SMI could inhibit the elevation in ST-segment. The hemodynamic parameters, left ventricular function and myocardial ischemic injury were visibly improved. The levels of CK, LDH and MDA activities and the heart index were significantly decreased. The level of SOD activity was obviously increased (p<0.05 or p<0.01). [Conclusion] SMI has some protective effects against myocardial ischemia injury induced by isoproterenol in rats.