蒙药胃舒安对束缚-水浸应激致胃溃疡的预防作用及对TGF-β1表达的影响

Prophylactic effect ofMongolian drug Wei Shu An on gastric ulcer induced by restraint and water immersion and effect on the expression of TGF-β1

关键词:  蒙药胃舒安|束缚-水浸应激|胃溃疡|TGF-β₁

Key words:  Mongolian drug Wei Shu An|restraint and water immersion stress|stomach ulcer|TGF-β₁

摘要:

[目的] 评价蒙药胃舒安对束缚-水浸应激所致大鼠胃溃疡的预防作用。[方法] 将Wistar实验大鼠，分正常组、给药组和对照组3组，正常组不给药，无束缚-水浸应激压力，对照组给淀粉，给药组灌胃给蒙药胃舒安，给药剂量为0.9~1.3mL/只（82.5g/L），连续给胃舒安7d后，停食24h，建立3h束缚-水浸应激性胃溃疡模型，取胃，肉眼观察胃黏膜损伤，用ImageJ图像分析法测定胃黏膜溃疡面积，并进行比较。用酶联免疫吸附测定法检测胃溃疡大鼠血清转化生长因子（TGF-β₁）含量并进行比较。[结果] 蒙药胃舒安对3h束缚-水浸应激所致大鼠胃溃疡形成有抑制作用。胃舒安组大鼠血清TGF-β₁含量明显低于对照组。[结论] 蒙药胃舒安对实验大鼠束缚-水浸应激3h所致胃溃疡的形成有显著抑制作用。

Abstract:

[Objective] To observe the prophylactic effect of Mongolian drug Wei Shu An on gastric ulcer induced by restraint and water immersion. [Methods] Male Wistar rats were divided into normal,treatment and control group. In normal group, no medicine and stress of water immersion were given, while in control group starch powder was received. After continuum oral administration of Mongolian drug Wei Shu An for 7 days a model of gastric ulcer induced with restraint and water immersion was established after stopping feeding for 24 h. At the end of experiment the stomach sample was taken. The gastric mucosa and the degree of injury were observed by naked eye. The ulcer area of the gastric mucosa was analyzed by Image J software and compared. The serum contents of transforming growth factor-β₁ (TGF-β₁) in rats with gastric ulcer was measured with enzyme-linked immunosorbent assay and compared. [Results] Mongolian drug Wei Shu An could inhibit the formation of gastric ulcer induced by restraint and water immersion stress for 3 h in rats. The content of serum TGF-β₁ in Wei Shu An group were significantly lower than that in the control group. [Conclusion] Mongolian drug Wei Shu An has an inhibiting effect on gastric ulcer induced by restraint and water immersion stress for 3 h in rats.