鳖甲寡肽I-C-F-6对乙醇诱导的小鼠慢性肝损伤的实验研究

Experimental study on the effect of oligo-peptide I-C-F-6 in Carapax Trionycis against chronic alcoholic liver injury in mice

关键词:  鳖甲寡肽I-C-F-6  慢性酒精性肝损伤  保护作用

Key words:  oligo-peptide I-C-F-6  chronic alcoholic liver injury  protective effect

摘要:

[目的] 探讨鳖甲寡肽I-C-F-6对乙醇诱导的小鼠慢性肝损伤的预防作用,并分析其可能的保护机制.[方法] 将90只ICR小鼠随机分为正常对照组、模型组、寡肽低剂量组(0.12 mg/kｇ)、寡肽高剂量组(0.24 mg/kg)、阳性药组(２40.00 mg/kｇ).正常对照组和模型组均皮下注射生理盐水(０.12 mL/kｇ);寡肽低剂量组和寡肽高剂量组分别皮下注射寡肽的生理盐水溶液(0.12 mL/kg);阳性药组灌胃还原型谷胱甘肽生理盐水溶液(０.12 mL/kｇ),同时除正常对照组外,其余4组给予灌胃40%乙醇(０.12 mL/kｇ),每日1次,连续16周.16周后,末次给乙醇12 h后处死小鼠采集血液样品和肝组织样品,测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)和碱性磷酸酶(AKP)的活力,肝组织超氧化物歧化酶(SOD)活力、丙二醛含量(MDA),单胺氧化酶(MAO)的活力,并进行病理组织学检查.[结果] 鳖甲寡肽I-C-F-6使灌胃乙醇后小鼠体内ALT、AST、MDA、MAO、AKP的含量显著降低(P<0.05),肝组织匀浆中SOD的活力明显升高(P<0.05),肝脏病理损伤减轻.[结论] 鳖甲寡肽I-C-F-6对小鼠慢性酒精性肝损伤具有一定的预防保护作用,其机制可能与抑制脂质过氧化,清除自由基,升高SOD的活力有关.

Abstract:

[Objective] To study the protective effect of oligo-peptide I-C-F-6 against chronic alcohol hepatic fibrosis in mice and to explore its possible mechanisms. [Methods] The ICR mice were randomly divided into five groups: normal control group, model group, oligo-peptide low dose group (0.12 mg/kg), oligo-peptide high dose group (0.24 mg/kg), positive drug group (240.00 mg/kg). Mice in oligo-peptide groups were administered oligo-peptide' saline solution (0.12 mL/kg) once a day, while mice in normal group and model group were given saline solution (0.12 mL/kg); positive drug group was given reduced-glutathione (0.12 mL/kg) by intragastric administration once a day. At the same time, in addition to the normal control group, the rest of the four groups were given 40% alcohol once a day, for 16 weeks. After 16 weeks, they were sacrificed for collecting the blood samples and liver tissue samples. At last, the serum alanine aminotransferase (ALT), aspertate aminotransferase (AST) and alkaline phosphatase (AKP) level and superoxide dismutase (SOD) in liver tissue and malondialdehyde (MDA) and monoamne oxidase (MAO) activity content were determined respectively. The changes of pathohisology of mice were observed. [Results] ALT, AST, AKP in mice, MAO, MDA content was significantly higher in chronic alcoholic liver injury model group. Compared with model group, the serum concentrations of ALT, AST, AKP and the liver tissue content of MDA and MAO were significantly decreased in treatment groups (P<0.05); the activity of SOD in the liver tissue in treatment groups were also significantly increased (P<0.05). Chronic alcoholic liver injury was significantly improved in treatment groups as compared with the model group. [Conclusion] Oligo-peptide I-C-F-6 has significant therapeutic effect on chronic alcoholic liver injury in mice. Its mechanism may be related to the inhibition of lipid peroxidation, scavenging free radicals and increasing the vitality of SOD.