升清降浊方对MSG肥胖大鼠脂代谢的影响及机制研究

Effects of Shengqing Jiangzhuo formula to improve the insulin sensitivity of MSG rats

关键词:  升清降浊方  L-谷氨酶钠肥胖大鼠  脂代谢

Key words:  Shengqing Jiangzhuo formula  MSG rat  lipid mechanism

摘要:

[目的] 研究升清降浊方(SQJZF)对L-谷氨酸钠(MSG)肥胖大鼠脂代谢的影响及其机制研究。[方法] 复制MSG肥胖大鼠模型,随机分为模型组、阳性药罗格列酮组、阳性药非诺贝特组、SQJZF高剂量组(含生药6 g/kg)、SQJZF中剂量组(含生药3 g/kg)、SQJZF低剂量组(含生药1.5 g/kg);另取正常组大鼠8只。连续给药50 d后,分别检测大鼠肝脏脂质和血清游离脂肪酸的含量;实时定量聚合酶链反应(PCR)检测药物对MSG大鼠肝脏中过氧化物酶体增殖物激活受体α(PPAR α)、脂蛋白脂肪酶(LPL)、载脂蛋白A-V(ApoA-V)、载脂蛋白C-Ⅲ(ApoC-Ⅲ)的基因表达水平。[结果] 模型组MSG大鼠肝脏总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)和血清游离脂肪酸(NEFA)含量显著升高(P<0.05或P<0.01),肝脏高密度脂蛋白(HDL-C)含量显著降低(P<0.01);肝脏PPARα、LPL基因的相对表达量降低,ApoA-V基因的相对表达量显著降低(P<0.01),ApoC-Ⅲ基因的相对表达量升高;给药50 d后,各给药组肝脏TC、TG、LDL-C和NEFA含量降低(P<0.05或P<0.01),肝脏HDL-C含量升高(P<0.05或P<0.01);对相关基因的相对表达均有一定的调节作用。[结论] SQJZF对MSG大鼠可能通过激活PPAR 受体调控脂蛋白代谢来控制MSG肥胖大鼠脂代谢紊乱。

Abstract:

[Objective] To study the mechanisms and effects of Shengqing Jiangzhuo formula(SQJZF) to improve the lipid metabolism of MSG rats. [Methods] Copy the MSG obese rat models, which were randomly divided into model group, rosiglitazone group, fenofibrate group, three dose of SQJZF. Then choose 8 normal rats as the normal control group. Measure the levels of liqid in liver and the level of NEFA in serum after administration for 50 days. Real-time PCR detect the expression level of PPAR, LPL, ApoA-V, A poC-Ⅲ in liver. [Results] Compared with the normal group, the model group improved the level of TC, TG, LDL-C in liver and the level of NEFA in serum and decreased the level of HDL-C in liver significantly (P<0.05 or P<0.01), and decreased the expression of PPAR, LPL, ApoA-V significantly (P<0.01), and improved the expression of ApoC-Ⅲ. Compared with the model group, each of groups had some improvements of the level of liqid in liver and the level of NEFA and the level of genes expression. [Conclusion] SQJZF improve the lipid metabolism disorders in MSG rats which by activating the genes expression of PPAR.