补阳还五汤对家兔易损斑块内新生血管的影响研究

Research the mechanism of Buyang Huanwu decoction on angiogenesis in vulnerable plaque in rabbits

关键词:  动脉粥样硬化  易损斑块  新生血管  补阳还五汤

Key words:  atherosclerosis  vulnerable plaques  angiogenesis  Buyang Huanwu decoction

摘要:

[目的] 研究补阳还五汤对兔动脉粥样硬化斑块内新生血管的影响及机制。[方法] 选取50只雄性新西兰白兔为研究对象,将其随机分为假手术组、模型组、补阳还五汤组,12周后处死。取斑块组织苏木精-伊红(HE)染色评估斑块破裂出血情况;取冰冻斑块组织,采用免疫荧光染色,评估血管密度情况;采用酶联免疫吸附(ELISA)及蛋白印记测定血中及斑块内碱性成纤维生长因子(bFGF)、血管内皮生长因子(VEGF)、血小板源性生长因子(PDGF)表达情况。[结果] 补阳还五汤组斑块破裂数明显少于模型组(P<0.05)斑块内bFGF、PDGF表达与模型组比较有统计学差异(P<0.05)。[结论] 补阳还五汤具有稳定易损斑块的作用,其机制可能与增加斑块内bFGF、PDGF表达,促进血管成熟有关。

Abstract:

[Objective] To explore the mechanism of Buyang Huanwu decoction(BYHWD) on angiogenes in vulnerable plaque in rabbits. [Methods] The vulnerable plaque model was reproduced by operation on abdominal aorta endothelium for inducing sacculus pull injury with the foraging with high cholesterol lipid feeding. Rabbits were divided into three groups: model group, sham-operation group and BYHWD group. The experiment lasted for 12 weeks. After killing at the end of experiment, the samples of abdominal aorta were collected for HE staining to accounting the number of rupture plaques, using immunofluorescence staining to evaluate the density of microvessels, detecting the protein content of VEGF, bFGF, PDGF in plaque and evaluating the content of VEGF, bFGF, PDGF in the serum of peripheral blood. [Results] It was found that the number of plaque rupture in BYHWD group was obviously less than model group by HE observation(P<0.05).The expression of bFGF, PDGF in plaque in BYHWD was statistically significant(P<0.05), compared with model group and sham-operated group. [Conclusion] BYHWD can stable the vulnerable plaque, and the mechanism may be related to increasing the expression of bFGF, PDGF and then promoting the microvessels maturation in plaque.